Targeting Enolase in Reducing Secondary Damage in Acute Spinal Cord Injury in Rats

Neurochemical Research
Azizul HaqueNaren L Banik

Abstract

Spinal cord injury (SCI) is a complex debilitating condition leading to permanent life-long neurological deficits. The complexity of SCI suggests that a concerted multi-targeted therapeutic approach is warranted to optimally improve function. Damage to spinal cord is complicated by an increased detrimental response from secondary injury factors mediated by activated glial cells and infiltrating macrophages. While elevation of enolase especially neuron specific enolase (NSE) in glial and neuronal cells is believed to trigger inflammatory cascades in acute SCI, alteration of NSE and its subsequent effects in acute SCI remains unknown. This study measured NSE expression levels and key inflammatory mediators after acute SCI and investigated the role of ENOblock, a novel small molecule inhibitor of enolase, in a male Sprague-Dawley (SD) rat SCI model. Serum NSE levels as well as cytokines/chemokines and metabolic factors were evaluated in injured animals following treatment with vehicle alone or ENOblock using Discovery assay. Spinal cord samples were also analyzed for NSE and MMPs 2 and 9 as well as glial markers by Western blotting. The results indicated a significant decrease in serum inflammatory cytokines/chemokines and NSE, al...Continue Reading

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Citations

Oct 24, 2019·Bioscience Reports·Cai-Ming XuHai-Long Chen
Jun 18, 2020·Headache·Agneta H SnoerRigmor H Jensen
Jan 27, 2019·Scientific Reports·Haaglim ChoDarren R Williams
Feb 16, 2021·Frontiers in Genetics·Frankis A AlmaguelCarlos A Casiano
Oct 15, 2020·International Journal of Molecular Sciences·Hyo Young JungIn Koo Hwang
Apr 18, 2020·Experimental Neurology·Azizul HaqueNaren L Banik

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Methods Mentioned

BETA
ELISA

Software Mentioned

ENOblock
Image J

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