Targeting Glioblastoma Cells Expressing CD44 with Liposomes Encapsulating Doxorubicin and Displaying Chlorotoxin-IgG Fc Fusion Protein

International Journal of Molecular Sciences
Hafizah MahmudMasaharu Seno

Abstract

We recently have established a successful xenograft model of human glioblastoma cells by enriching hyaluronic acid-dependent spheroid-forming populations termed U251MG-P1 cells from U251MG cells. Since U251MG-P1 cells have been confirmed to express CD44 along with principal stemness marker genes, OCT3/4, SOX2, KLF4 and Nanog, this CD44 expressing population appeared to majorly consist of undifferentiated cells. Evaluating the sensitivity to anti-cancer agents, we found U251MG-P1 cells were sensitive to doxorubicin with IC50 at 200 nM. Although doxorubicin has serious side-effects, establishment of an efficient therapy targeting undifferentiated glioblastoma cell population is necessary. We previously designed a chlorotoxin peptide fused to human IgG Fc region without hinge sequence (M-CTX-Fc), which exhibited a stronger growth inhibitory effect on the glioblastoma cell line A172 than an original chlorotoxin peptide. Combining these results together, we designed M-CTX-Fc conjugated liposomes encapsulating doxorubicin and used U251MG-P1 cells as the target model in this study. The liposome modified with M-CTX-Fc was designed with a diameter of approximately 100-150 nm and showed high encapsulation efficiency, adequate loading cap...Continue Reading

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Citations

Mar 2, 2019·International Journal of Molecular Sciences·Apriliana Cahya KhayraniMasaharu Seno
Oct 9, 2018·Journal of Drug Targeting·Shoshy MizrahyDan Peer
Aug 20, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Srishti AgarwalD Sakthi Kumar
Dec 1, 2018·Nano Convergence·You Jung KangHansang Cho
Jun 19, 2019·Cell Communication and Signaling : CCS·Sharon McGonigleKenichi Nomoto
Apr 25, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Salman Ul IslamYoung Sup Lee
Dec 5, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sepideh MirzaeiManoj Garg
May 15, 2021·Biotechnology Advances·Julien GiribaldiChristina I Schroeder
Jun 7, 2021·International Journal of Pharmaceutics·Marcela Tavares LuizMarlus Chorilli

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Methods Mentioned

BETA
surgical resection
xenograft
electrophoresis

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