Targeting liver sinusoidal endothelial cells with miR-20a-loaded nanoparticles reduces murine colon cancer metastasis to the liver

International Journal of Cancer. Journal International Du Cancer
Joana MarquezIker Badiola

Abstract

Phenotypic transformation of liver sinusoidal endothelial cells is one of the most important stages of liver metastasis progression. The miRNA effects on liver sinusoidal endothelial cells during liver metastasis have not yet been studied. Herein, whole genome analysis of miRNA expression in these cells during colorectal liver metastasis revealed repressed expression of microRNA-20a. Importantly, downregulation of miR-20a occurs in parallel with upregulation of its known protein targets. To restore normal miR-20a levels in liver sinusoidal endothelial cells, we developed chondroitin sulfate-sorbitan ester nanoparticles conjugated with miR-20a in a delivery system that specifically targets liver sinusoidal endothelial cells. The restoration of normal mir-20a levels in these cells induced downregulation of the expression of its protein targets, and this also resulted in a reduction of in vitro LSEC migration and a reduction of in vivo activation and tumor-infiltrating capacity and ability of the tumor decreased by ∼80% in a murine liver metastasis model.

References

Oct 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P A McCourtS Johansson
Feb 26, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Elvira OlasoFernando Vidal-Vanaclocha
Jun 17, 2006·Cell·Suvendra N BhattacharyyaWitold Filipowicz
Oct 24, 2007·Particle and Fibre Toxicology·Evaldas SadauskasGorm Danscher
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Apr 11, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Edward N HarrisPaul H Weigel
Oct 21, 2009·Progress in Molecular and Subcellular Biology·Marc R FabianNahum Sonenberg
Jun 1, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Beatriz ArtetaFernando Vidal-Vanaclocha
Nov 3, 2010·Biochimica Et Biophysica Acta·Shannon A BaxterJeffrey T Wigle
May 17, 2011·Journal of Translational Medicine·Clarisa SaladoFernando Vidal-Vanaclocha
Mar 22, 2014·Journal of Proteome Research·Gereon PoschmannKai Stühler
May 27, 2014·PloS One·Yin XiongElectron Kebebew
May 27, 2014·Advanced Drug Delivery Reviews·Yunching ChenLeaf Huang
Sep 28, 2014·International Journal of Pharmaceutics·A PensadoA Sanchez
Jul 3, 2015·Cold Spring Harbor Perspectives in Biology·Kathryn N Ivey, Deepak Srivastava
Aug 19, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alexandra C RinkenauerUlrich S Schubert
Nov 13, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Kamesh R Babu, Martina U Muckenthaler
Dec 1, 2015·International Journal of Pharmaceutics·E AndréC N Montero-Menei
May 21, 2016·Cancer Research·Stephanie LangschErik Vassella
May 26, 2016·Cancer Prevention Research·Ester BorrasEduardo Vilar
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hui MaoYajun Guo
May 31, 2016·International Journal of Molecular Sciences·Zuhair HawaSushanta K Banerjee
May 31, 2016·International Journal of Molecular Sciences·Dan-Dan CaoWai-Yee Chan

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Citations

Sep 24, 2019·Journal of Cellular Biochemistry·Yiping ZhuXiaoping Qian
Oct 1, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Usman ArshadAndrew Owen
Apr 16, 2020·Cells·Erica LafozVirginia Hernández-Gea
Sep 29, 2020·Frontiers in Physiology·Alex L WilkinsonShishir Shetty
Oct 3, 2018·Molecular Medicine Reports·Jianyu FanYu Zhang
Jul 28, 2020·Nanomaterials·Chunhua Yang, Didier Merlin
Oct 31, 2019·Journal of Oncology·Riccardo RampadoMarco Agostini
May 20, 2018·Journal of Experimental & Clinical Cancer Research : CR·Junfeng SunJiaxiang Wang
Oct 10, 2020·Journal of Experimental & Clinical Cancer Research : CR·Jian LiQingling Zhang
Dec 15, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Shusuke TodenAjay Goel
Oct 29, 2020·Cellular & Molecular Immunology·Andrea Kristina HorstGisa Tiegs
Apr 21, 2020·Current Pharmaceutical Biotechnology·Atieh Hashemi, Gilar Gorji-Bahri
Jul 6, 2021·World Journal of Clinical Cases·Yvette UmwaliXin Zhang
Mar 23, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yun Hong, Yuefeng Rao
Mar 13, 2020·Molecular Therapy. Nucleic Acids·Jaitip TipaneeThierry VandenDriessche

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