Targeting MALAT1 and miRNA-181a-5p for the intervention of acute lung injury/acute respiratory distress syndrome.

Respiratory Medicine
Yaling LiuHong Xie

Abstract

ALI/ARDS is a severe lung injury leading to refractory respiratory failure, accounting for high morbidity and mortality. However, therapeutic approaches are rather limited. Targeting long non-coding RNA MALAT1 and microRNA miR-181a-5p might be potential option for ALI/ARDS intervention. We aimed to investigate the role of MALAT and miR-181a-5p in the pathogenesis of ALI/ARDS, and test the therapeutic effects of targeting MALAT and miR-181a-5p for ALI/ARDS intervention in vitro. MALAT1 and miR-181a-5p levels were measured in plasma from ALI/ARDS patients. In vitro human pulmonary microvascular endothelial cell (HPMEC) injury was induced by LPS treatment, and molecular targets of MALAT1 and miR-181a-5p were explored by molecular biology approaches, mainly focusing on cell apoptosis and vascular inflammation. Interaction between MALAT1 and miR-181a-5p was also detected. Finally, the effects of targeting MALAT1 and miR-181a-5p for ALI/ARDS intervention were validated in a rat ALI/ARDS model. MALAT1 upregulation and miR-181a-5p downregulation were observed in ALI/ARDS patients. Transfection of mimic miR-181a-5p into HPMECs revealed decreased Fas and apoptosis, along with reduced inflammatory factors. Fas was proved to be a direct ta...Continue Reading

References

Sep 24, 2004·Annals of Internal Medicine·Claude A Piantadosi, David A Schwartz
Jan 13, 2005·Critical Care Medicine·Polly E ParsonsUNKNOWN NHLBI Acute Respiratory Distress Syndrome Clinical Trials Network
Jun 30, 2007·American Journal of Respiratory and Critical Care Medicine·Mario PerlAlfred Ayala
Jan 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Frank SchembriAvrum Spira
Feb 8, 2011·Biochimica Et Biophysica Acta·Jürgen SchellerStefan Rose-John
Mar 8, 2014·Circulation Research·Katharina M MichalikStefanie Dimmeler
Mar 29, 2014·TheScientificWorldJournal·Rituparno SenJayprokas Chakrabarti
Dec 3, 2014·The Surgical Clinics of North America·Julie Ottosen, Heather Evans
Jan 31, 2015·Seminars in Liver Disease·Ashley M Mohr, Justin L Mott
Mar 15, 2015·BMC Genomics·Janice DuyTimothy D Minogue
Mar 20, 2015·Journal of Cellular and Molecular Medicine·Prasanth PuthanveetilSubrata Chakrabarti
May 1, 2015·Respiration; International Review of Thoracic Diseases·Charles SharpAndrew R L Medford
Oct 30, 2016·European Journal of Pharmacology·Shamila D AlipoorAliakbar Velayati
Jun 16, 2017·The Journal of Biological Chemistry·Nicholas W Mathy, Xian-Ming Chen
Oct 5, 2017·Clinical Medicine : Journal of the Royal College of Physicians of London·Christopher MasonMark Griffiths
Nov 15, 2017·Current Opinion in Critical Care·Bairbre A McNicholasJohn G Laffey
Feb 13, 2018·Annals of Translational Medicine·Raquel HerreroJose Angel Lorente
Mar 27, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Guoping WangYuyou Zhu
Mar 29, 2018·Respiratory Research·Erica K Y Fan, Jie Fan
Apr 29, 2018·Archives of Biochemistry and Biophysics·Hongbin LiRongqing Sun
May 22, 2018·American Journal of Respiratory and Critical Care Medicine·Elizabeth A Middleton, Guy A Zimmerman
Nov 6, 2018·Thorax·Raquel HerreroJosé Ángel Lorente

❮ Previous
Next ❯

Citations

Jun 12, 2021·Journal of Cellular and Molecular Medicine·Jun LiJiangzhou Peng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis