Targeting of AUF1 to vascular endothelial cells as a novel anti-aging therapy

Journal of Geriatric Cardiology : JGC
Jian HeYong-Xiang Zhao

Abstract

Inhibition of aging of vascular endothelial cells (VECs) may delay aging and prolong life. The goal of this study was to prepare anti-CD31 monoclonal antibody conjugated PEG-modified liposomes containing the AU-rich region connecting factor 1 (AUF1) gene (CD31-PILs-AUF1) and to explore the effects of targeting CD31-PILs-AUF1 to aging VECs. The mean particle sizes of various PEGylated immunoliposomes (PILs) were measured using a Zetasizer Nano ZS. Gel retardation assay was used to confirm whether PILs had encapsulated the AUF1 plasmid successfully. Fluorescence microscopy and flow cytometry were used to quantify binding of CD31-PILs-AUF1 to target cells. Flow cytometry was also used to analyze the cell cycles of aging bEnd3 cells treated with CD31-PILs-AUF1. We also developed an aging mouse model by treating mice with D-galactose. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). The malondialdehyde (MDA) and the superoxide dismutase (SOD) levels were detected by commercial kits. Hematoxylin-eosin (HE) staining was used to determine whether treatment with CD31-PILs-AUF1 was toxic to the mice. CD31-PILs-AUF1 specifically could targeted bEnd3 VECs...Continue Reading

Citations

Dec 24, 2018·American Journal of Physiology. Cell Physiology·Yong LanYang-Fang Li
Dec 23, 2021·Experimental and Therapeutic Medicine·Daojiang YuShuyu Zhang

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Methods Mentioned

BETA
Enzyme-linked immunosorbent assay
fluorescence microscopy
Assay
flow cytometry
MDA
ELISA

Software Mentioned

GraphPad Prism
Malvern Zetasizer
Flowjo

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