Targeting of endogenous sulfated glycoprotein-1 and -2 to lysosomes within nonciliated cells of the efferent ducts during postnatal development of the rat

Molecular Reproduction and Development
L HermoC R Morales

Abstract

Sulfated glycoprotein (SGP)-1 and -2, secretory products of Sertoli cells, are secreted into the lumen of seminiferous tubules where they bind to late spermatids. Once released, the spermatozoa traverse the efferent ducts where these proteins detach from their surface and are endocytosed by the nonciliated cells. In adult animals, SGP-1 and SGP-2 are also synthesized by nonciliated cells and targeted from the Golgi apparatus to lysosomes. The purpose of the present study was to determine the pattern of expression of SGP-1 and SGP-2 within nonciliated cells during postnatal development. The efferent ducts of animals at different postnatal ages were prepared for an electron microscopic immunocytochemical quantitative analysis as well as for Northern blot analysis. The data expressed as labeling content (no. gold particles/micron 2 and taking into account the volume of the endocytic organelles and the cell) revealed that anti-SGP-1 labeling in endosomes of nonciliated cells was minimal at 15, 21, and 29 days of age. On the other hand, the lysosomal labeling content showed a significant increase by day 29 compared to 15 and 21-day-old animals indicating that an endogenous form of SGP-1 was being synthesized by nonciliated cells and...Continue Reading

References

Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M HiraiwaJ S O'Brien
Mar 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J S O'Brien, Y Kishimoto
Jun 1, 1970·Endocrinology·D W KnorrM B Lipsett
Sep 1, 1984·The American Journal of Anatomy·L Hermo, C Morales
Dec 15, 1994·Microscopy Research and Technique·M H Stoffel, A E Friess
Dec 15, 1994·Microscopy Research and Technique·K Y Ilio, R A Hess

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Citations

Oct 31, 2003·Biology of Reproduction·Matthew D AnwayLouis Hermo
Sep 4, 2008·Reproductive Biomedicine Online·Chitra Lekha DahiaA Jagannadha Rao
Jul 18, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C C LuedtkeL Hermo
Nov 27, 2020·Cell and Tissue Research·Tetsuya ShimokawaSeiji Matsuda

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