PMID: 9435217Jan 22, 1998Paper

Targeting of GroEL to SecA on the cytoplasmic membrane of Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
E BochkarevaA Girshovich

Abstract

Chaperonin GroEL has been found to interact with isolated cytoplasmic membrane of Escherichia coli. Interaction requires Mg ions, whereas MgATP inhibits, and inhibition is stronger in the presence of co-chaperonin GroES. "Heat-shock" of the membrane at 45 degrees C destroys irreversibly its ability to bind GroEL. The binding of GroEL is characterized by saturation with a maximum of about 100 pmol GroEL bound per mg of total membrane protein, indicating a limited capacity and specificity of the membrane to bind GroEL. According to results of immunoblotting analysis and cleavable photoactivable cross-linking, a membrane target of GroEL is SecA, a protein known as a central component of the translocation machinery. Moreover, in some cases GroEL could modulate a cycle of association of SecA with the membrane by stimulating release of SecA from the membrane. A physiological role of targeting of GroEL in or close to the protein-conducting membrane apparatus is discussed.

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Citations

Jan 5, 2000·Microbes and Infection·M G Schmidt, K B Kiser
Oct 7, 2010·The Journal of Biological Chemistry·David WickströmJan-Willem de Gier
Nov 2, 2004·Journal of Bacteriology·Adam C Fisher, Matthew P DeLisa
Jul 21, 2005·Journal of Bacteriology·Gabija ZiedaiteDennis H Bamford
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·H TjalsmaJ M van Dijl
Oct 26, 2000·Cell Stress & Chaperones·J Kim, D A Kendall
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·John DeatonRy Young
Apr 20, 2001·Molecular Microbiology·J W de Gier, J Luirink
Jan 25, 2012·Journal of Applied Microbiology·G SeydlováJ Svobodová
Feb 28, 2006·Research in Microbiology·Maria PlesaPierre Cornelis
Jul 5, 2005·Expert Opinion on Therapeutic Targets·A Economou
Nov 26, 2013·Biochimica Et Biophysica Acta·Marie-Pierre Castanié-CornetPierre Genevaux
Jun 19, 2016·Journal of Biotechnology·Haixia ChiFang Huang
Oct 2, 2002·FEBS Letters·Galit Levy-RimlerAbdussalam Azem
Aug 14, 2019·Frontiers in Microbiology·Maria S LoosAnastassios Economou
Aug 4, 1999·Trends in Microbiology·A Economou
Nov 12, 1998·Trends in Biochemical Sciences·L VighJ L Harwood
May 1, 2021·Frontiers in Molecular Biosciences·Chen JiangDamon Huber
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·P Fekkes, A J Driessen
Feb 15, 2003·Biochimica Et Biophysica Acta·David DrewJan-Willem L de Gier
Jun 11, 2021·PloS One·Jessica RauchAnke Osterloh
Apr 13, 2005·Journal of Proteome Research·Renu NandakumarJulia M Ross

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