Targeting of microRNA-21-5p protects against seizure damage in a kainic acid-induced status epilepticus model via PTEN-mTOR

Epilepsy Research
Chongyang TangZhiguo Lin

Abstract

Studies have shown that microRNAs play a role in the development of epilepsy by regulating downstream target messenger (m)RNA. The present study aims to determine the changes associated with microRNA-21-5p (miR-21-5p) during epileptogenesis in a kainic acid rat model, and to assess whether the PTEN-mTOR pathway is a target of miR-21-5p. Reverse transcription polymerase chain reaction (RT-PCR) was used to examine the quantitative expressions of miR-21-5p and PTEN, and Western blotting was used to test the activity of mTOR in the acute, latent, and chronic stages of epileptogenesis. The antagomir of miR-21-5p was injected into the intracerebroventricular space using a microsyringe. Neuronal death and epilepsy discharge were assessed by Nissl staining and electroencephalography (EEG), respectively. The Morris water maze (MWM) was used to assess the cognitive impairment in rats after status epilepticus (SE). Both miR-21-5p and mTOR were upregulated and PTEN was downregulated in rats during acute, latent, and chronic stages of epileptogenesis when compared with those of the control. After using antagomir miR-21-5p in vivo, miR-21-5p and mTOR decreased and the expression of PTEN increased compared with that in the SE model. The silen...Continue Reading

Citations

May 22, 2019·Advances in Nutrition·Jae Young KwonSang Ryong Kim
Feb 23, 2019·Cellular and Molecular Neurobiology·Chongyang TangZhiguo Lin
Jun 26, 2020·Proceedings of the National Academy of Sciences of the United States of America·Morten T VenøDavid C Henshall
Apr 28, 2020·Computers in Biology and Medicine·Shuhan YangFengfeng Zhou
Sep 21, 2020·European Journal of Pharmacology·Dezaree Raut, Lokesh Kumar Bhatt

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