Targeting of sebocytes by aminolevulinic acid-dependent photosensitization

Photochemistry and Photobiology
Sachiko KosakaBernhard Ortel

Abstract

Photodynamic therapy using 5-aminolevulinic acid-induced protoporphyrin IX has been developed as a very useful therapeutic modality. Recently, several authors have reported on the efficacy of this procedure for acne. This approach is based on the fact that 5-aminolevulinic acid-induced protoporphyrin IX has strong selectivity for sebaceous glands. We used the immortalized human sebaceous gland cell line SZ95 to investigate cellular mechanisms of photodynamic therapy using 5-aminolevulinic acid-induced protoporphyrin IX. Quantification of induced protoporphyrin IX production showed dependence on the applied 5-aminolevulinic acid dose. When SZ95 sebocytes were differentiated by arachidonic acid treatment, there was no difference between them and the control cells with respect to both the amount of 5-aminolevulinic acid-induced protoporphyrin IX and the phototoxic effects. We altered protoporphyrin IX formation rates by growing cells scattered as single cells in the culture dishes. Single cells produced significantly lower protoporphyrin IX levels than those grown with intercellular contacts. Intracellular localization of protoporphyrin IX was imaged using confocal laser scanning microscopy. The differentiation-specific lipid drop...Continue Reading

References

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Citations

Jun 4, 2008·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Agustí TollRamon M Pujol
Sep 25, 2008·Photodermatology, Photoimmunology & Photomedicine·Cristina ZanePiergiacomo Calzavara-Pinton
May 14, 2008·Experimental Dermatology·Christos C ZouboulisAnja Thielitz
Jul 28, 2011·Medicinal Research Reviews·Frederick Harris, Lynne Pierpoint
Jan 1, 2015·Nature Reviews. Disease Primers·Sara Moradi TuchayiChristos C Zouboulis
Sep 6, 2012·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Pier Giacomo Calzavara-PintonUNKNOWN Italian Group For Photodynamic Therapy

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Methods Mentioned

BETA
FCS
fluorescence imaging

Software Mentioned

SAS

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