Targeting of Swi/Snf to the yeast GAL1 UAS G requires the Mediator, TAF IIs, and RNA polymerase II

The EMBO Journal
Karine Lemieux, Luc Gaudreau

Abstract

The chromatin remodeling activity of the Swi/Snf complex is essential for the expression of several yeast genes. Previous studies have suggested that recruitment of Swi/Snf requires the action of transcriptional activators. However, reports in metazoans and in yeast have provided evidence of interactions between Swi/Snf and the RNA polymerase II holoenzyme/Mediator complex. Here we show that recruitment of Swi/Snf to the galactose-inducible gene GAL1 cannot be fully achieved without the integrity of the Mediator complex, TAF IIs, and RNA polymerase II. Moreover, artificial recruitment of Mediator is sufficient to tether both Swi/Snf and SAGA to the GAL1 UAS G. We further demonstrate that Swi/Snf recruitment at GAL1 does not require acetylation of chromatin by Gcn5 nor the presence of SAGA. Based on these results, we conclude that interactions between the Gal4 activator and Swi/Snf are not sufficient to recruit the latter to the GAL1 UAS G, since interactions with the Mediator, TAF IIs, and RNA polymerase II are also important.

References

May 25, 1992·Nucleic Acids Research·S R HaynesI B Dawid
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·C M Thompson, R A Young
Feb 29, 1996·Nature·J QuinnC L Peterson
Sep 15, 1996·Genes & Development·S FarrellM Ptashne
Dec 27, 1996·Cell·B R CairnsR D Kornberg
May 1, 1997·Trends in Biochemical Sciences·F JeanmouginR Losson
Oct 29, 1997·Molecular and Cellular Biology·K J Pollard, C L Peterson
Jan 10, 1998·Trends in Genetics : TIG·M Hampsey
Mar 14, 1998·Nucleic Acids Research·A S NeishJ D Parvin
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J CôtéJ L Workman
Jul 14, 1998·Molecular Cell·S S KohR A Young
Aug 26, 1998·Molecular and Cellular Biology·H ChoD Reinberg
Dec 9, 1998·Cell·F C HolstegeR A Young
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·L GaudreauM Ptashne
Apr 16, 1999·The EMBO Journal·S R Biggar, G R Crabtree
Sep 29, 1999·Genes & Development·N YudkovskyC L Peterson
Jan 19, 2000·Trends in Biochemical Sciences·C E BrownJ L Workman
Feb 25, 2000·Molecular and Cellular Biology·M VignaliJ L Workman
Apr 4, 2000·Nature·P SyntichakiG Thireos
Dec 2, 2000·Nature Genetics·M PrimigR E Esposito
Jun 8, 2001·Annual Review of Biochemistry·S Y RothC D Allis
Jul 5, 2001·Cellular and Molecular Life Sciences : CMLS·K HavasT Owen-Hughes
Aug 4, 2001·Genes & Development·S R Bhaumik, M R Green

❮ Previous
Next ❯

Citations

Feb 4, 2005·Chromosoma·Erik BlazekMichael Meisterernst
Oct 8, 2011·ACS Chemical Biology·Malathy KrishnamurthyAnna K Mapp
Apr 18, 2007·Genes & Development·Sharmistha KunduCraig L Peterson
Oct 31, 2007·Molecular and Cellular Biology·Xiaohui LiuErnest Martinez
Jun 21, 2005·Molecular and Cellular Biology·Chhabi K GovindAlan G Hinnebusch
Apr 16, 2005·Molecular and Cellular Biology·Hongfang QiuAlan G Hinnebusch
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Sarah MacPhersonBernard Turcotte
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Amy SvotelisLuc Gaudreau
Oct 6, 2005·Biological Chemistry·Anton EberharterPeter Becker
Oct 9, 2007·Journal of Molecular Biology·Mei Kee LimNorbert Lehming
May 4, 2006·EMBO Reports·Ana TravenMary Sopta
Feb 19, 2015·Nature Reviews. Molecular Cell Biology·Benjamin L Allen, Dylan J Taatjes
Aug 26, 2011·Seminars in Cell & Developmental Biology·David Ries, Michael Meisterernst
Oct 25, 2011·Trends in Genetics : TIG·Xin WangMark Ptashne
May 18, 2018·Molecular Biology of the Cell·Elisa DultzKarsten Weis
Apr 20, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Q Tian Wang
Aug 19, 2015·Molecular and Cellular Biology·Alessandro RienzoMarkus Proft
Feb 18, 2016·Molecular and Cellular Biology·Xu ChenLaurie A Stargell
Nov 20, 2013·Molecular and Cellular Biology·Suting ZhengJoseph C Reese
May 28, 2005·Yeast
Mar 31, 2009·Yeast·Rhiannon Biddick, Elton T Young
Apr 28, 2010·Nature Reviews. Genetics·Vikki M Weake, Jerry L Workman
Sep 6, 2012·MicrobiologyOpen·Avery G Frey, David J Eide
Jan 31, 2013·Cellular and Molecular Life Sciences : CMLS·Suraiya A Ansari, Randall H Morse
Dec 21, 2007·The Journal of Biological Chemistry·Qiye HeRandall H Morse
Oct 21, 2006·Biochemical Society Transactions·M K Leverentz, R J Reece
Feb 26, 2016·Cell & Bioscience·Aleksey N KrasnovNadezhda E Vorobyeva
Nov 11, 2015·BMC Developmental Biology·Xin LouIan C Scott
Nov 7, 2019·Cell Death & Disease·Nadine H BrückmannMorten F Gjerstorff
Nov 11, 2020·International Journal of Molecular Sciences·Amparo Pascual-AhuirMarkus Proft
Feb 13, 2007·Mutation Research·Mary Ann OsleyJac A Nickoloff
Feb 20, 2007·Mutation Research·Cassandra Hogan, Patrick Varga-Weisz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.