Targeting Plk1 to chromosome arms and regulating chromosome compaction by the PICH ATPase

Cell Cycle
Mei LengJun Qin

Abstract

During mitosis, chromosomes undergo dynamic structural changes that include condensation of chromosomes-the formation of individual compact chromosomes necessary for faithful segregation of sister chromatids in anaphase. Polo-like kinase 1 (Plk1) regulates multiple mitotic events by binding to targeting factors at different mitotic structures in a phosphorylation dependent manner. In this study, we report the identification of a putative ATPase that targets Plk1 to chromosome arms during mitosis. PICH (Plk1-interacting checkpoint "helicase") displays a temporal localization on chromosome arms and kinetochores during early mitosis. Interaction with PICH recruits Plk1 to chromosome arms and disruption of this interaction abolishes Plk1 localization on chromosome arms. Moreover, depletion of PICH or overexpression of PICH mutant that is defective in Plk1 binding or ATP binding causes defects in mitotic chromosome compaction, formation of anaphase bridge and cytokinesis failure. We provide data to show that both PICH phosphorylation and its ATPase activity are required for mitotic chromosome compaction. Our study provides a mechanism for targeting Plk1 to chromosome arms and suggests that the PICH ATPase activity is important for t...Continue Reading

Citations

Apr 8, 2011·International Journal of Hematology·Songmin Ying, Ian D Hickson
Feb 5, 2010·Molecular Biology of the Cell·Yasuhiro Kurasawa, Li-yuan Yu-Lee
Dec 9, 2015·Nature Communications·Christian F NielsenIan D Hickson
Oct 21, 2014·The Plant Journal : for Cell and Molecular Biology·Tomomi HaraTsukaho Hattori
Aug 27, 2013·Molecular Cell·Andreas BiebricherIan D Hickson
Nov 24, 2017·Cold Spring Harbor Symposia on Quantitative Biology·Kata SarlósIan D Hickson
Jan 8, 2015·The Journal of Biological Chemistry·Vinidhra SridharanYoshiaki Azuma
Jul 11, 2019·Oncology Letters·Yang XieJian Qi
Dec 14, 2018·Genes·María Fernández-Casañas, Kok-Lung Chan
Feb 11, 2010·Journal of Cell Science·Lily Hui-Ching WangErich A Nigg

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