Targeting relaxase genes for classification of the predominant plasmids in Enterobacteriaceae

International Journal of Medical Microbiology : IJMM
Fabrice CompainDominique Decré

Abstract

Plasmids are the main vectors of antimicrobial drug resistance and virulence genes, especially in Enterobacteriaceae. Identification and classification of plasmids is essential for analysis of their distribution. The most widely used typing method is PCR-based replicon typing (PBRT). A new classification scheme based on relaxase gene typing has been described recently. We propose a practical application of this method, with the development of a multiplex PCR set targeting relaxase genes found on plasmids most frequently encountered in Enterobacteriaceae. This method, here called "plasmid relaxase gene typing" (PRaseT), was validated with 60 transconjugants and transformants harboring various replicon types. The method was tested with 39 multidrug-resistant clinical isolates including Escherichia coli, Klebsiella pneumoniae and Salmonella enterica subsp. enterica carrying 1-7 replicons as well as with 17 plasmids non-typeable using PBRT; all replicons were tested in parallel with PBRT for comparison. Six multiplex PCRs and one simplex PCR, including 24 pairs of primers, recognized plasmids of groups A/C, B/O, colE, FIA, FIB, FIC, FV, FIIk, HI1, HI2, I1, K, L/M, N, P1α, Q1, U, W, X1, X2, X3 and X4. There was perfect correlation b...Continue Reading

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Citations

Jul 24, 2014·Diagnostic Microbiology and Infectious Disease·Fabrice CompainCharlotte Verdet
Aug 18, 2016·Emerging Infectious Diseases·Laure SurgersDominique Decré
Jan 26, 2018·The Journal of Antimicrobial Chemotherapy·M RozwandowiczJ Hordijk
Feb 18, 2016·Antimicrobial Agents and Chemotherapy·Abdoul-Salam OuédraogoSylvain Godreuil
Jul 15, 2015·Antimicrobial Agents and Chemotherapy·Salim AberkaneSylvain Godreuil
Aug 13, 2014·Antimicrobial Agents and Chemotherapy·M A JureD Decré
Jun 22, 2017·Emerging Microbes & Infections·Fabrice CompainDominique Decré
Aug 14, 2019·Antimicrobial Agents and Chemotherapy·Oumar Ouchar MahamatSylvain Godreuil
Aug 19, 2017·Emerging Infectious Diseases·Yahia BenzeraraGuillaume Arlet
Jan 26, 2017·The Journal of Antimicrobial Chemotherapy·Marion DuprilotGuillaume Arlet
Apr 25, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Amel AbderrahimAlix Pantel
Mar 26, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Nadia ChaalalAlix Pantel
Aug 3, 2018·Clinical Microbiology Reviews·Sally R PartridgeSlade O Jensen
Jan 12, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Nadia ChaalalAlix Pantel
Nov 3, 2020·Frontiers in Microbiology·Elizabeth A McMillanJonathan G Frye
Nov 6, 2018·International Journal of Medical Microbiology : IJMM·Laure SurgersDominique Decré

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