Abstract
Human adenoviruses are non-enveloped DNA viruses causing various infections; their pathogenicity varies dependent on virus species and type. Although acute infections can sometimes take severe courses, they are rarely fatal in immune-competent individuals. Adenoviral conjunctivitis and epidemic keratoconjunctivitis are hyperacute and highly contagious infections of the eye caused by human adenovirus types within species D. Currently there is no causal treatment available to counteract these diseases effectively. The E2B region of the adenovirus genome encodes for the viral DNA polymerase, which is required for adenoviral DNA replication. Here we propose novel model systems to test this viral key factor, DNA polymerase, as a putative target for the development of efficient antiviral therapy based on RNA interference. Using our model cell lines we found that different small interfering RNAs mediate significant suppression (up to 90%) of expression levels of viral DNA polymerase upon transfection. Moreover, permanent expression of short hairpin RNA based on the most effective small interfering RNA led to a highly significant, more than tenfold reduction in replication for different human group D adenoviruses involved in ocular inf...Continue Reading
References
Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·N J CaplenR A Morgan
Apr 16, 2003·Journal of Medical Virology·Albert HeimPatricia Pring-Akerblom
Jun 25, 2004·Nucleic Acids Research·Bingbing YuanFran Lewitter
Sep 27, 2005·Bone Marrow Transplantation·G D MyersC M Bollard
Aug 8, 2006·Journal of Clinical Microbiology·Karin EbnerThomas Lion
Mar 26, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kristoffer WeberBoris Fehse
May 13, 2008·BMC Genomics·Christopher M RobinsonJames Chodosh
Jul 26, 2008·Reviews in Medical Virology·Liesbeth LenaertsLieve Naesens
Dec 17, 2008·Current Gene Therapy·Oded Singer, Inder M Verma
Apr 4, 2009·Advanced Drug Delivery Reviews·N ManjunathPremlata Shankar
Dec 18, 2009·Gene Therapy·K WeberB Fehse
Feb 16, 2011·Drugs·Chrysanthi L SkevakiAthanassios Tsakris
May 5, 2011·International Journal of Obesity : Journal of the International Association for the Study of Obesity·H-N NaJ-H Nam
Feb 3, 2012·Molekuliarnaia biologiia·P V SpirinV S Prasolov
Apr 6, 2012·Journal of Clinical Gastroenterology·Guglielmo M TrovatoDaniela Catalano
Apr 19, 2012·Antiviral Research·Doris KneidingerReinhard Klein
Apr 21, 2012·Emerging Infectious Diseases·Yuki MatsushimaHiroshi Ushijima
Jul 10, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Susanna EspositoNicola Principi
Apr 15, 2014·Leukemia·P V SpirinV S Prassolov
Oct 15, 2014·Journal of Diabetes and Its Complications·Olga DubuissonNikhil V Dhurandhar
Oct 31, 2014·World Journal of Gastroenterology : WJG·Francesca M TrovatoGuglielmo M Trovato
Dec 6, 2014·PloS One·Ha-Na NaJae-Hwan Nam