Targeting the Notch1 oncogene by miR-139-5p inhibits glioma metastasis and epithelial-mesenchymal transition (EMT)

BMC Neurology
Jianlong LiRuiyan Li

Abstract

Glioma metastasis, invasion, epithelial-mesenchymal transition (EMT) and chemoresistance indicate poor prognosis. Accumulating evidence reveals that Notch1 is an important factor in tumour progression. However, the role of Notch1 in glioma EMT and associated microRNAs (miRNAs) with the Notch pathway remain controversial. Utilizing cBioPortal database to examine the gene signature of NOTCH1 (encoding Notch1), CDH2 (encoding N-cadherin) and SNAI1 (encoding Snail-1) in disease-free survival (DFS) and overall survival (OS). We analyzed the Notch1 expression from Oncomine. We used Western blot (WB), immunohistochemistry (IHC) and immunofluorescence to determine protein levels. Transcription was evaluated by quantitative real-time (qRT)-PCR. siRNA and lentivirus were used to knock down Notch1 and overexpress miR-139-5p, respectively. The migration and invasion of glioma cells were assessed by wound healing and transwell assays. Luciferase reporter assays were utilized to verify the relationship between Notch1 and miR-139-5p. A U87-implanted intracranial model was used to study the effect of miR-139-5p on tumour growth and Notch1 suppression efficacy or EMT reversion. It revealed the association of NOTCH1, CDH2, SNAI1 genomic alterati...Continue Reading

References

Apr 17, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·A GieseM Westphal
Aug 10, 2007·Cellular and Molecular Life Sciences : CMLS·U Koch, F Radtke
Feb 4, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Zhi-Hua ZhuTie-Hua Rong
Dec 15, 2010·Nature Reviews. Clinical Oncology·Naoko TakebeS Percy Ivy
Jan 13, 2011·The EMBO Journal·Simone BrabletzThomas Brabletz
Sep 21, 2012·Journal of Neurology, Neurosurgery, and Psychiatry·Alex TobiasMaciej S Lesniak
Apr 4, 2013·Science Signaling·Jianjiong GaoNikolaus Schultz
Apr 5, 2013·CNS Neuroscience & Therapeutics·Rui-Yan LiChuan-Lu Jiang
May 25, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Guanhua XieAnna Mae Diehl
Mar 20, 2014·British Journal of Cancer·H ZhangY Sun
Apr 3, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sihai YueYong Li
Feb 13, 2016·Technology in Cancer Research & Treatment·Hong WangSan-Zhong Li
May 14, 2016·Pathology, Research and Practice·Heyong LiuZhaohui Huang
Jul 2, 2016·Cell·M Angela NietoJean Paul Thiery
Nov 30, 2016·Journal of Experimental & Clinical Cancer Research : CR·Jianlong LiChuanlu Jiang
Dec 10, 2016·Blood Cancer Journal·K KrowiorzF Kuchenbauer
Dec 7, 2017·Disease Models & Mechanisms·Jessica A BlaquiereEsther M Verheyen

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Citations

Apr 15, 2020·International Journal of Molecular Sciences·Carlos DeOcesano-PereiraMari Cleide Sogayar
Sep 3, 2020·Cancer Biotherapy & Radiopharmaceuticals·Dan LeiBo Zhang
Aug 8, 2019·Pathology, Research and Practice·Sheng XinXin-Gen Zhu
Dec 3, 2020·Cancer Management and Research·Xiang Wen YuanHuilin Tong
Feb 15, 2021·Molecular and Cellular Biochemistry·Anahita EbrahimpourSadra Samavarchi Tehrani
Jul 19, 2020·Clinical Oncology : a Journal of the Royal College of Radiologists·K Thippu Jayaprakash, A Michael

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Methods Mentioned

BETA
surgical resection
PCR
electrophoresis
transfection
xenograft

Software Mentioned

miRanda
Image pro - plus
Targetscan
SPSS
GraphPad Prism

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