Targeting tryptophan and tyrosine metabolism by liquid chromatography tandem mass spectrometry

Journal of Chromatography. a
Josep MarcosOscar J Pozo

Abstract

An imbalance in tryptophan (Trp) and tyrosine (Tyr) metabolites is associated with neurological and inflammatory disorders. The accurate and precise measurement of these compounds in biological specimens is a powerful tool to understand the biochemical state in several diseases. In this study, a rapid, accurate and sensitive method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the targeted analysis of the metabolism of Trp and Tyr has been developed and validated. The method allows for the adequate quantification of Trp, Tyr and, eight Trp metabolites, three Tyr metabolites, together with four competitive large neutral amino acids. Serotonin, 5-hydroxyindoleacetic acid, kynurenine, kynurenic acid, dopamine, and homovanilic acid were among the targeted compounds. Sample preparation, chromatographic separation and mass spectrometric detection were optimized in human urine, human plasma and mice prefrontal cortex extracts. The method was shown to be linear (r>0.98) in the range of endogenous concentrations for all studied metabolites. In general, the limits of detection were suitable for the detection of the endogenous levels. Intra- and inter-assay precisions below 25% and accuracies ranging from 80 to 12...Continue Reading

References

Jun 1, 1978·Journal of Neurochemistry·J D Fernstrom, D V Faller
Aug 5, 1975·Biochimica Et Biophysica Acta·W M Pardridge, W H Oldendorf
Jan 1, 1997·Journal of Psychopharmacology·J G ReillyA H Young
Aug 7, 1998·Journal of Psychopharmacology·P Schloss, D C Williams
May 5, 1999·Journal of Neural Transmission·H BaranL Deecke
Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·I P KemaF A Muskiet
Oct 16, 2001·Biological Psychiatry·R SchwarczR C Roberts
Mar 22, 2003·The Journal of Pharmacology and Experimental Therapeutics·David S GoldsteinIrwin J Kopin
Sep 14, 2004·Mental Retardation and Developmental Disabilities Research Reviews·Diane C Chugani
May 18, 2005·Journal of Nutritional Science and Vitaminology·Tsutomu FtukijwatariKatsumi Shibata
Sep 2, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Luana LionettoMaurizio Simmaco
Feb 7, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wilhelmina H A de JongIdo P Kema
Feb 9, 2010·Analytical and Bioanalytical Chemistry·Wilhelmina H A de JongIdo P Kema
Sep 10, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Sujatha KannanDiane C Chugani
Aug 23, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jianxing ZhaoDingliang Zhu
Dec 14, 2011·Analytical and Bioanalytical Chemistry·Mikaela I NichkovaGottfried H Kellermann
Jan 18, 2012·The FEBS Journal·Trevor W StoneL Gail Darlington
Nov 15, 2012·International Journal of Tryptophan Research : IJTR·Chiaki HiratsukaKatsumi Shibata
Feb 21, 2013·Neuropharmacology·Christian KleinAyikoe G Mensah-Nyagan
Sep 17, 2013·Analytical Biochemistry·Wojciech G LesniakRangaramanujam M Kannan
Oct 8, 2014·International Journal of Tryptophan Research : IJTR·Magdalena E KegelSophie Erhardt

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Citations

Apr 8, 2017·Clinica Chimica Acta; International Journal of Clinical Chemistry·Catharina M KorseHuub H van Rossum
Mar 22, 2018·Bioanalysis·Jose Rodríguez-MoratóJosep Marcos
Jul 6, 2018·Journal of Periodontal Research·H W ChenZ C Song
Nov 20, 2018·Journal of Psychopharmacology·Eulàlia OlestiRafael de la Torre
Mar 21, 2019·Biomedical Chromatography : BMC·Jun DengJing Li
Jan 19, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marine P M LetertreIan D Wilson
May 30, 2020·Journal of Pharmaceutical and Biomedical Analysis·Ana Sofia Abrantes DiasBruno Manadas
Apr 17, 2021·Journal of Inherited Metabolic Disease·Jordi To-FiguerasSverre Sandberg
May 6, 2021·International Journal of Molecular Sciences·Judith Marín-CorralOscar J Pozo

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