Targeting type II and Clara cells for adenovirus-mediated gene transfer using the surfactant protein B promoter

American Journal of Respiratory Cell and Molecular Biology
M S StrayerP L Ballard

Abstract

We examined the ability of the human surfactant protein B (SP-B) promoter to confer cell specificity of transgene expression in an adenoviral vector. Using similar replication-deficient adenoviruses (rAd), we compared lacZ reporter gene expression driven by the human SP-B promoter (rAd.SPBlacZ) with the ubiquitously expressed Rous sarcoma virus promoter (rAd.RSVlacZ). rAd.SPBlacZ expressed lacZ in H-441 and A549 lung epithelial cell lines and not in HeLa cells whereas rAd.RSVlacZ expressed in all three cell lines. In primary human fetal lung fibroblasts, beta-galactosidase activity from rAd.RSVlacZ transduction increased in a dose-dependent manner whereas activity from rAd.SPBlacZ remained low. In mixed cell cultures prepared from human fetal lung explants that contained fibroblasts and type II cells, X-Gal staining localized rAd.SPBlacZ expression to only type II cells whereas rAd.RSVlacZ expressed in both cell types. In 24-wk gestation human fetal tissue explants infected ex vivo, the RSV promoter directed lacZ expression in lung, trachea, heart, liver, and esophagus, whereas with the SP-B promoter lacZ was expressed only in lung, specifically in air space-lining cells. This specificity was maintained in vivo. lacZ expression...Continue Reading

References

Oct 1, 1992·American Journal of Respiratory Cell and Molecular Biology·M F BeersA B Fisher
Oct 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M T StahlmanJ A Whitsett
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T R KorfhagenJ A Whitsett
Jul 1, 1989·Laboratory Animals·B Starcher, I Williams
Sep 4, 1986·Biochimica Et Biophysica Acta·P L BallardM C Williams
Apr 1, 1986·The Journal of Clinical Endocrinology and Metabolism·L W GonzalesM C Williams
Jan 1, 1980·Cell and Tissue Research·R J PackJ G Widdicombe
Apr 1, 1995·The American Journal of Physiology·D K VorbrokerJ A Whitsett
Apr 1, 1995·The American Journal of Physiology·V C VenkateshP L Ballard
Jun 1, 1994·American Journal of Respiratory Cell and Molecular Biology·R R MercerJ D Crapo
Aug 1, 1994·American Journal of Respiratory Cell and Molecular Biology·D E deMelloH R Colten
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·M G KaplittD W Pfaff
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·J L BessereauJ P Changeux
Oct 1, 1993·American Journal of Respiratory Cell and Molecular Biology·C E HarrisD T Curiel
Feb 11, 1993·The New England Journal of Medicine·L M NogeeH R Colten
May 2, 1996·The New England Journal of Medicine·J M Wilson
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·W S SimonetJ A Whitsett
Jul 1, 1996·The American Journal of Physiology·E A MensahJ S Lwebuga-Mukasa
Jan 1, 1997·American Journal of Respiratory Cell and Molecular Biology·J C ClarkJ A Whitsett

❮ Previous
Next ❯

Citations

Jul 21, 1999·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·K S FrederiksenH S Poulsen
Jul 11, 2006·Biochemistry. Biokhimii︠a︡·E M GlinkaS M Deyev
Aug 31, 2011·Pulmonary Medicine·Jeffrey A KazzazMarla R Wolfson
Jun 28, 2011·Critical Care : the Official Journal of the Critical Care Forum·James DevaneyJohn G Laffey
May 24, 2012·International Journal of Nanomedicine·Paul ZarogoulidisKonstantinos Zarogoulidis
Apr 30, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gabriele ToiettaArthur L Beaudet
Sep 5, 1998·The American Journal of Physiology·D SalvailE Rousseau
Sep 5, 1998·The American Journal of Physiology·M DumoulinE Rousseau
Dec 8, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hshi-Chi KooJeffrey A Kazzaz
Nov 22, 2000·American Journal of Physiology. Heart and Circulatory Physiology·J E GravesW A Large
Feb 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Marlene StrayerPhilip L Ballard
Jul 17, 1999·The American Journal of Physiology·J L AschnerD W Busija

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.