tarsal-less is expressed as a gap gene but has no gap gene phenotype in the moth midge Clogmia albipunctata

Royal Society Open Science
Eva Jimenez-GuriJohannes Jaeger

Abstract

Gap genes are involved in segment determination during early development of the vinegar fly Drosophila melanogaster and other dipteran insects (flies, midges and mosquitoes). They are expressed in overlapping domains along the antero-posterior (A-P) axis of the blastoderm embryo. While gap domains cover the entire length of the A-P axis in Drosophila, there is a region in the blastoderm of the moth midge Clogmia albipunctata, which lacks canonical gap gene expression. Is a non-canonical gap gene functioning in this area? Here, we characterize tarsal-less (tal) in C. albipunctata. The homologue of tal in the flour beetle Tribolium castaneum (called milles-pattes, mlpt) is a bona fide gap gene. We find that Ca-tal is expressed in the region previously reported as lacking gap gene expression. Using RNA interference, we study the interaction of Ca-tal with gap genes. We show that Ca-tal is regulated by gap genes, but only has a very subtle effect on tailless (Ca-tll), while not affecting other gap genes at all. Moreover, cuticle phenotypes of Ca-tal depleted embryos do not show any gap phenotype. We conclude that Ca-tal is expressed and regulated like a gap gene, but does not function as a gap gene in C. albipunctata.

References

Sep 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·M StauberU Schmidt-Ott
Apr 19, 2007·PLoS Biology·Máximo Ibo GalindoJuan Pablo Couso
May 9, 2007·Nature Cell Biology·Takefumi KondoYuji Kageyama
Jul 22, 2008·Evolution & Development·Steffen LemkeUrs Schmidt-Ott
Sep 20, 2008·Developmental Biology·Jose Ignacio Pueyo, Juan Pablo Couso
May 1, 2010·Development·Steffen LemkeUrs Schmidt-Ott
May 4, 2010·Developmental Biology·Mónica García-SolacheMichael Akam
Oct 12, 2010·Cellular and Molecular Life Sciences : CMLS·Johannes Jaeger
Apr 30, 2011·Developmental Biology·Jose I Pueyo, Juan Pablo Couso
Feb 26, 2013·BMC Genomics·Eva Jiménez-GuriJohannes Jaeger
Jan 11, 2014·PloS One·Eva Jiménez-GuriJohannes Jaeger
Feb 12, 2014·Nature Reviews. Genetics·Shea J Andrews, Joseph A Rothnagel
Aug 2, 2017·Annual Review of Cell and Developmental Biology·Serge PlazaFrançois Payre
Nov 22, 2017·EvoDevo·Karl R WottonEva Jiménez-Guri

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Datasets Mentioned

BETA
MG783326

Methods Mentioned

BETA
PCR

Software Mentioned

Ca
FlyAGE
FlyGUI
tal

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