Task-based imaging of colon cancer in the Apc(Min/+) mouse model.

Applied Optics
James B McNallyJennifer K Barton

Abstract

Optical coherence tomography (OCT), laser-induced fluorescence (LIF), and laser-scanning confocal microscopy (LSCM) were used for the task of multimodal study of healthy and adenomatous mouse colon. The results from each modality were compared with histology, which served as the gold standard. The Apc(Min/+) genetic mouse model of colon cancer was compared with wild-type mice. In addition, a special diet was used for the task of studying the origins of a 680 nm autofluorescent signal that was previously observed in colon. The study found close agreement among each of the modalities and with histology. All four modalities were capable of identifying diseased tissue accurately. The OCT and LSCM images provided complementary structural information about the tissue, while the autofluorescence signal measured by LIF and LSCM provided biochemical information. OCT and LIF were performed in vivo and nondestructively, while the LSCM and histology required extraction of the tissue. The magnitude of the 680 nm signal correlates with chlorophyll content in the mouse diet, suggesting that the autofluorescent compound is a dietary metabolite.

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Citations

Sep 13, 2006·Journal of Biomedical Optics·Elizabeth M KanterJennifer K Barton
Nov 3, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Amy M WinklerJennifer K Barton
Jan 4, 2008·ILAR Journal·Isabel J HildebrandtWolfgang A Weber
Aug 30, 2008·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·C Chad QuarlesJ Oliver McIntyre
Aug 26, 2014·BioMed Research International·Aileen P PattersonReuben Saba
Jul 30, 2010·Tissue Engineering. Part C, Methods·Chao-Wei ChenYu Chen
Aug 18, 2017·Journal of Biophotonics·Helene IslerAlexander Kalyanov
Mar 20, 2019·Journal of Cancer Research and Clinical Oncology·Junze RenBai Li
Mar 18, 2011·Biomedical Optics Express·R Andrew WallJennifer K Barton
Dec 20, 2019·Biomedical Optics Express·Changsik YoonJannick P Rolland
Nov 10, 2017·Cancer Prevention Research·Photini F S RiceNatalia A Ignatenko
Jun 29, 2021·ILAR Journal·Kaela L NavarroCholawat Pacharinsak

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