TAT-BH4 and TAT-Bcl-xL peptides protect against sepsis-induced lymphocyte apoptosis in vivo

The Journal of Immunology : Official Journal of the American Association of Immunologists
Richard S HotchkissDavid Piwnica-Worms

Abstract

Apoptosis is a key pathogenic mechanism in sepsis that induces extensive death of lymphocytes and dendritic cells, thereby contributing to the immunosuppression that characterizes the septic disorder. Numerous animal studies indicate that prevention of apoptosis in sepsis improves survival and may represent a potential therapy for this highly lethal disorder. Recently, novel cell-penetrating peptide constructs such as HIV-1 TAT basic domain and related peptides have been developed to deliver bioactive cargoes and peptides into cells. In the present study, we investigated the effects of sepsis-induced apoptosis in Bcl-x(L) transgenic mice and in wild-type mice treated with an antiapoptotic TAT-Bcl-x(L) fusion protein and TAT-BH4 peptide. Lymphocytes from Bcl-x(L) transgenic mice were resistant to sepsis-induced apoptosis, and these mice had a approximately 3-fold improvement in survival. TAT-Bcl-x(L) and TAT-BH4 prevented Escherichia coli-induced human lymphocyte apoptosis ex vivo and markedly decreased lymphocyte apoptosis in an in vivo mouse model of sepsis. In conclusion, TAT-conjugated antiapoptotic Bcl-2-like peptides may offer a novel therapy to prevent apoptosis in sepsis and improve survival.

References

Sep 1, 1995·The Journal of Experimental Medicine·D T ChaoS J Korsmeyer
Aug 14, 1999·Critical Care Medicine·R S HotchkissI E Karl
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·R S HotchkissI E Karl
Mar 23, 2001·Nature Immunology·R S HotchkissI E Karl
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R S HotchkissI E Karl
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·C OberholzerL L Moldawer
Feb 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Richard S HotchkissIrene E Karl
Apr 3, 2002·JAMA : the Journal of the American Medical Association·Craig M CoopersmithRichard S Hotchkiss
Oct 3, 2002·Molecular and Cellular Neurosciences·Gunnar P H DietzMathias Bähr
Oct 29, 2002·Annals of Neurology·Ertugrul KilicMathias Bähr
Dec 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sadamitsu AsohShigeo Ohta
May 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Richard S HotchkissIrene E Karl
May 28, 2003·Trends in Pharmacological Sciences·Catherine Denicourt, Steven F Dowdy
Sep 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Akiko IwataRobert K Winn
Nov 25, 2003·Oncogene·Rie SugiokaYoshihide Tsujimoto
May 29, 2004·Shock·Philip A EfronLyle L Moldawer
Jul 31, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joel G R WeaverAndrew D Badley
Oct 16, 2004·Molecular and Cellular Neurosciences·Gunnar P H Dietz, Mathias Bähr
Oct 20, 2004·American Journal of Clinical Pathology·Paolo TotiGiuseppe Buonocore
Dec 29, 2004·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Masamichi OnoHikaru Matsuda
Mar 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yufei WangThomas Lehner
Mar 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kate A FelmetJoseph A Carcillo
Apr 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Richard S HotchkissIrene E Karl
Jun 9, 2005·Blood·Doreen E Wesche-SoldatoAlfred Ayala

❮ Previous
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Citations

Jan 9, 2007·Annals of Surgical Oncology·Hiroyuki KashiwagiWilliam G Hawkins
Jan 24, 2007·Physiological Reviews·Guido KroemerCatherine Brenner
Sep 15, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jonathan E McDunnRichard S Hotchkiss
Feb 29, 2008·American Journal of Physiology. Renal Physiology·Hideo YasudaRobert A Star
Mar 20, 2010·Molecular BioSystems·Rupa Sawant, Vladimir Torchilin
Nov 5, 2016·Apoptosis : an International Journal on Programmed Cell Death·Thibaut GirardotGuillaume Monneret
May 4, 2017·Immunological Reviews·Kun-Po LiDavid A Hildeman
Sep 9, 2017·The Journal of Experimental Medicine·Matthias KollekMiriam Erlacher
May 16, 2018·Cell Death Discovery·Tim VervlietGeert Bultynck
Apr 14, 2010·Shock·Kevin R KastenCharles C Caldwell
Oct 20, 2009·Nature Cell Biology·Jong-Soo LeeJae U Jung
Nov 21, 2014·Cell Death & Disease·M AzizP Wang
Jan 25, 2017·Nature Reviews. Disease Primers·Richard S HotchkissJean-Louis Vincent
Apr 7, 2016·Cellular and Molecular Life Sciences : CMLS·Stefan KrautwaldUlrich Kunzendorf
May 1, 2008·Personalized Medicine·Derek S Wheeler, Hector R Wong
Mar 15, 2018·Frontiers in Immunology·David S Ucker, Jerrold S Levine
May 22, 2019·Cold Spring Harbor Perspectives in Biology·Clark W Distelhorst, Martin D Bootman
Jan 20, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tracey H WagnerJ Perren Cobb
May 24, 2008·The Journal of Clinical Investigation·Toshio HisatomiJoan W Miller
Feb 24, 2010·Nature Reviews. Nephrology·Anna GlubaJacek Rysz
Jun 9, 2007·Circulation Research·Tong Tang, H Kirk Hammond
May 1, 2009·International Journal of Cancer. Journal International Du Cancer·Peter O SimonWilliam G Hawkins
Mar 31, 2015·BioMed Research International·Xia FanHua-ping Liang
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yi-Ping RongClark W Distelhorst
Mar 5, 2016·Cell Death and Differentiation·D S Ucker
Nov 6, 2007·Pharmacogenomics·Hector R Wong
Oct 14, 2006·Nature Reviews. Immunology·Richard S Hotchkiss, Donald W Nicholson
Jul 18, 2008·Journal of the American Society of Nephrology : JASN·Ana Belen SanzAlberto Ortiz
Jul 27, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Prajwal GurungThomas S Griffith
May 23, 2015·Expert Opinion on Drug Delivery·Peter Lönn, Steven F Dowdy
Feb 17, 2017·Carcinogenesis·Chiara GabelliniDonatella Del Bufalo
Oct 5, 2019·Expert Opinion on Drug Delivery·Kimia KardaniAzam Bolhassani
Mar 7, 2020·Pharmaceutics·Rebecca E Taylor, Maliha Zahid
May 8, 2007·Emerging Infectious Diseases·Janie ParrinoMike Bray
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Steven J SchwulstRichard S Hotchkiss

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