TATA-binding protein is limiting for both TATA-containing and TATA-lacking RNA polymerase III promoters in Drosophila cells.

Molecular and Cellular Biology
A TrivediDeborah L Johnson

Abstract

We have investigated the role of the TATA-binding protein (TBP) in modulating RNA polymerase (Pol) III gene activity. Epitope-tagged TBP (e-TBP) was both transiently and stably transfected in Drosophila Schneider S-2 cells to increase the total cellular level of TBP. Analysis of the transcripts synthesized from cotransfected tRNA and U6 RNA genes revealed that both types of RNA Pol III promoters were substantially stimulated by an increase in e-TBP in a dose-dependent manner. Furthermore, a TBP-dependent increase in the levels of endogenous tRNA transcripts was produced in the stable line induced to express the e-TBP. We further determined whether the ability of increased TBP to induce RNA Pol III gene expression was due to a direct effect of increased TBP complexes on RNA Pol III gene promoters or an indirect consequence of enhanced expression of RNA Pol II genes. A TBP expression plasmid (e-TBP332), containing a mutation within the highly conserved carboxy-terminal domain, was both transiently and stably transfected into S-2 cells. e-TBP332 augmented the transcription from two RNA Pol II gene promoters indistinguishably from that observed when e-TBP was expressed. In contrast, e-TBP332 was completely defective in its ability ...Continue Reading

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Citations

May 2, 2007·The Plant Journal : for Cell and Molecular Biology·Chandra Prakash ChaturvediRakesh Tuli
May 15, 2007·Molecular Cell·Sandra S JohnsonDeborah L Johnson
Nov 18, 1998·Yeast·G H PattersonD W Piston
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Robert J White
Apr 25, 2013·Nature Reviews. Cancer·Megan J BywaterRoss D Hannan
Apr 17, 2003·Molecular and Cellular Biology·Sandra A S JohnsonDeborah L Johnson
Jun 24, 2000·Molecular and Cellular Biology·S A JohnsonD L Johnson
Nov 14, 1997·Genes & Development·A Ghavidel, M C Schultz

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