TATA-binding protein mutants that are lethal in the absence of the Nhp6 high-mobility-group protein

Molecular and Cellular Biology
Peter ErikssonDavid J Stillman

Abstract

The Saccharomyces cerevisiae Nhp6 protein is related to the high-mobility-group B family of architectural DNA-binding proteins that bind DNA nonspecifically but bend DNA sharply. Nhp6 is involved in transcriptional activation by both RNA polymerase II (Pol II) and Pol III. Our previous genetic studies have implicated Nhp6 in facilitating TATA-binding protein (TBP) binding to some Pol II promoters in vivo, and we have used a novel genetic screen to isolate 32 new mutations in TBP that are viable in wild-type cells but lethal in the absence of Nhp6. The TBP mutations that are lethal in the absence of Nhp6 cluster in three regions: on the upper surface of TBP that may have a regulatory role, near residues that contact Spt3, or near residues known to contact either TFIIA or Brf1 (in TFIIIB). The latter set of mutations suggests that Nhp6 becomes essential when a TBP mutant compromises its ability to interact with either TFIIA or Brf1. Importantly, the synthetic lethality for some of the TBP mutations is suppressed by a multicopy plasmid with SNR6 or by an spt3 mutation. It has been previously shown that nhp6ab mutants are defective in expressing SNR6, a Pol III-transcribed gene encoding the U6 splicing RNA. Chromatin immunoprecipit...Continue Reading

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Citations

Aug 16, 2011·The Journal of Biological Chemistry·Shinya TakahataDavid J Stillman
May 21, 2008·Molecular and Cellular Biology·Debabrata BiswasDavid J Stillman
Jul 11, 2007·Molecular and Cellular Biology·Debabrata BiswasDavid J Stillman
Sep 2, 2004·Molecular and Cellular Biology·Debabrata BiswasDavid J Stillman
Jul 1, 2005·Molecular and Cellular Biology·Debabrata BiswasDavid J Stillman
Oct 16, 2012·Biochimica Et Biophysica Acta·Joël AckerOlivier Lefebvre
Nov 21, 2012·Biochimica Et Biophysica Acta·Robyn D Moir, Ian M Willis
Oct 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·George A KassavetisE Peter Geiduschek
Aug 17, 2005·Current Opinion in Genetics & Development·Marco E Bianchi, Alessandra Agresti
Jun 2, 2009·Molecular Cell·Shinya TakahataDavid J Stillman
Dec 17, 2014·Molecular and Cellular Biology·Robert M YarringtonDavid J Stillman
Sep 16, 2006·The EMBO Journal·Debabrata BiswasDavid J Stillman
Sep 28, 2007·The EMBO Journal·Warren P VothDavid J Stillman
Jan 13, 2006·The Journal of Biological Chemistry·George A Kassavetis, David F Steiner
Oct 1, 2020·Nucleic Acids Research·Kiyoto KamagataReid C Johnson
Oct 4, 2020·Nucleic Acids Research·Yaxin YuDavid J Stillman
Jun 13, 2006·The Journal of Biological Chemistry·Danny Reinberg, Robert J Sims
Feb 23, 2008·The Journal of Biological Chemistry·Mohammed SbiaDavid J Stillman
Sep 12, 2018·Proceedings of the National Academy of Sciences of the United States of America·Robert M YarringtonDana Carroll

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