TatC-dependent translocation of pyoverdine is responsible for the microbial growth suppression

The Journal of Microbiology
Yeji LeeUn-Hwan Ha

Abstract

Infections are often not caused by a colonization of Pseudomonas aeruginosa alone but by a consortium of other bacteria. Little is known about the impact of P. aeruginosa on the growth of other bacteria upon coinfection. Here, cell-ree culture supernatants obtained from P. aeruginosa suppressed the growth of a number of bacterial strains such as Corynebacterium glutamicum, Bacillus subtilis, Staphylococcus aureus, and Agrobacterium tumefaciens, but had little effect on the growth of Escherichia coli and Salmonella Typhimurium. The growth suppression effect was obvious when P. aeruginosa was cultivated in M9 minimal media, and the suppression was not due to pyocyanin, a well-known antimicrobial toxin secreted by P. aeruginosa. By performing transposon mutagenesis, PA5070 encoding TatC was identified, and the culture supernatant of its mutant did not suppress the growth. HPLC analysis of supernatants showed that pyoverdine was a secondary metabolite present in culture supernatants of the wild-type strain, but not in those of the PA5070 mutant. Supplementation of FeCl2 as a source of iron compromised the growth suppression effect of supernatants and also recovered biofilm formation of S. aureus, indicating that pyoverdine-mediated...Continue Reading

References

Jan 1, 1992·Critical Reviews in Microbiology·B R OttoD M MacLaren
Sep 1, 1986·European Journal of Biochemistry·D WatsonG W Taylor
Jan 1, 1980·Journal of Bacteriology·H M Hassan, I Fridovich
Mar 1, 1995·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·L KiernanE Brown
Jul 1, 1993·Journal of Bacteriology·M T FollettieA J Sinskey
Oct 1, 1993·PCR Methods and Applications·G Caetano-Anollés
Feb 1, 1996·Infection and Immunity·J M MeyerI A Holder
Dec 31, 1997·Science·A M SettlesR Martienssen
Mar 18, 2000·Infection and Immunity·M HandfieldR C Levesque
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Iain L LamontMichael L Vasil
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Urs A OchsnerMichael L Vasil
Apr 3, 2004·Journal of Bacteriology·Valerie B Weaver, Roberto Kolter
Jan 22, 2005·Molecular Microbiology·Hemantha D KulasekaraStephen Lory
Mar 8, 2005·Research in Microbiology·Matthias Müller
Jul 1, 1986·Applied and Environmental Microbiology·L TorresJ P Beltrán
Sep 26, 2006·Applied and Environmental Microbiology·Yoshimi KikuchiLong-Fei Wu
Jul 2, 2009·Annual Review of Microbiology·Paul D Straight, Roberto Kolter
Jul 28, 2009·Journal of Bacteriology·Lalitha BiswasFriedrich Götz
Feb 4, 2010·Research in Microbiology·Itzel RamosDianne K Newman
May 25, 2012·Proteomics·Sabine KlugeUdo Reichl
Nov 14, 2012·Nature Reviews. Microbiology·Anders FolkessonSøren Molin
Oct 8, 2014·Nature Communications·Erwan PernetLhousseine Touqui

❮ Previous
Next ❯

Citations

Mar 18, 2017·Frontiers in Microbiology·Franziska S BirmesSusanne Fetzner
Aug 15, 2021·Molecular Ecology·Alexandre R T FigueiredoRolf Kümmerli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.