Tattoo Delivery of a Semliki Forest Virus-Based Vaccine Encoding Human Papillomavirus E6 and E7

Vaccines
Stephanie van de WallT Daemen

Abstract

The skin is an attractive organ for immunization because of the presence of antigen-presenting cells. Intradermal delivery via tattooing has demonstrated superior vaccine immunogenicity of DNA vaccines in comparison to conventional delivery methods. In this study, we explored the efficacy of tattoo injection of a tumor vaccine based on recombinant Semliki Forest virus replicon particles (rSFV) targeting human papillomavirus (HPV). Tattoo injection of rSFV particles resulted in antigen expression in both the skin and draining lymph nodes. In comparison with intramuscular injection, the overall antigen expression determined at the site of administration and draining lymph nodes was 10-fold lower upon tattoo injection. Delivery of SFV particles encoding the E6 and E7 antigens of human papillomavirus type 16 (SFVeE6,7) via tattooing resulted in HPV-specific cytotoxic T cells and in vivo therapeutic antitumor response. Strikingly, despite the observed lower overall transgene expression, SFVeE6,7 delivered via tattoo injection resulted in higher or equal levels of immune responses as compared to intramuscular injection. The intrinsic immunogenic potential of tattooing provides a benefit for immunotherapy based on an alphavirus.

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Citations

Nov 10, 2016·Vaccines·Kenneth Lundstrom
May 31, 2016·Current Opinion in Immunology·Jeffrey B Ulmer, Andrew J Geall
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Sep 20, 2020·Vaccines·Johanna Hettinga, Robert Carlisle

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Methods Mentioned

BETA
fluorescence microscopy
FACS
flow cytometry
FCS
ELISA
bioluminescence

Software Mentioned

Igor Pro
Elispot
Living Image

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