Taurine alleviates endoplasmic reticulum stress in the chondrocytes from patients with osteoarthritis

Redox Report : Communications in Free Radical Research
Yiqun BianShui Sun

Abstract

Osteoarthritis (OA), characterized by pain and stiffness, swelling, deformity and dysfunction of joints, affects large numbers of population. The purpose of this study was to discover the effects of taurine in human OA chondrocytes and explore the underlying mechanisms. 46 patients with different grades of OA were recruited. Of these patients, 24 underwent total knee replacement and cartilages were harvested. The mRNA expressions of type II collagen (Collagen II) and endoplasmic reticulum (ER) stress markers (GRP78, GADD153 and Caspase-12) in cartilages were quantified by qRT-PCR. Cell viability and apoptosis of patient-derived chondrocytes were assessed by the CCK-8 assay and flow cytometry assay, respectively. Meanwhile, protein levels of Collagen II and ER stress markers both in cartilages and chondrocytes were evaluated by Western blot. The mRNA and protein levels of Collagen II decreased as OA progressed, while the expressions of ER stress markers increased dramatically. H2O2 induced ER stress in chondrocytes, as shown by the significant increase in the expression of ER stress markers, inhibited chondrocyte viability and Collagen II synthesis, promoted apoptosis. However, taurine treatment inhibited these above phenomena. ...Continue Reading

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Citations

May 14, 2020·F1000Research·Michael D BriggsKatarzyna A Pirog
Dec 7, 2018·Dose-response : a Publication of International Hormesis Society·Jiangang CaoBo Li
Feb 11, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Magdalena KusaczukMarzanna Cechowska-Pasko
Aug 22, 2021·Calcified Tissue International·Rebecca F ShepherdAdam M Taylor
May 1, 2021·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Zubeyir ElmazogluÇimen Karasu

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Methods Mentioned

BETA
flow cytometry
PCR

Software Mentioned

SPSS

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis