Taurine attenuates lipopolysaccharide-induced disfunction in mouse mammary epithelial cells

Cytokine
Jinfeng MiaoSixiang Zou

Abstract

The intent of this study was to evaluate the active defense reaction of mouse mammary epithelial cells and the cytoprotective and anti-inflammatory properties of taurine to lipopolysaccharide (LPS)-induced disfunction in mouse mammary epithelial cells. (1) Primary cultured mouse mammary epithelial cells were stimulated with LPS for 24 h (final concentration=0, 5, 10, 20 μg/mL). Western blotting demonstrated a significant decrease in the secretion of β-casein in the 20 μg/mL LPS treatment group (P<0.05), while nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), lactoferrin (LF) and N-acetyl-β-D-glucosaminidase (NAGase) were all significantly increased following LPS treatment (P<0.01). Furthermore, cell survival was significantly inhibited after treatment with 20 μg/mL LPS; however, neither 5 μg/mL nor 10 μg/mL LPS had any effect on cell survival. Therefore, a level of 10 μg/mL LPS was selected to test the protective effect of taurine on mouse mammary epithelial cells. (2) Primary cultured mouse mammary epithelial cells were treated with 0, 5, 15 or 45 mmol/L taurine for 3 h, followed by 10 μg/mL LPS for 24 h. Taurine significantly attenuated the LPS-induced increase in NAGase activit...Continue Reading

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Citations

Sep 23, 2014·International Immunopharmacology·Wei ZhuSixiang Zou
May 31, 2013·International Immunopharmacology·Jinfeng MiaoLiuhai Zheng
Dec 3, 2014·The Journal of Veterinary Medical Science·Yueqiang WangJianfa Wang
Oct 21, 2016·The Journal of Biological Chemistry·Laura BouchéHoward R Morris
Jan 13, 2021·Cell and Tissue Research·Yusaku TsugamiKen Kobayashi

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