Taurine determination by capillary electrophoresis with laser-induced fluorescence detection: from clinical field to quality food applications

Amino Acids
Angelo ZinelluCiriaco Carru

Abstract

In this work we describe a new method for taurine quantification in plasma by capillary electrophoresis laser-induced fluorescence detection. Taurine is derivatized with fluorescein isothiocyanate at 100 degrees C in 20 min. These conditions allow to reduce the pre-analytical times and to derivatize quantitatively the taurine contained in the reaction mixture, contrary to the room temperature derivatization commonly adopted. FITC-taurine adduct is analyzed in an uncoated fused-silica capillary, 75 mum ID and 40 cm effective length using a 20 mmol/L tribasic sodium phosphate buffer pH 11.8, at 22 kV. To avoid the typical problems due to instability of FITC-adduct, we use the homocysteic acid as internal standard. The loss of FITC-taurine signal during the sequence analysis is compensated by the same loss of FITC-internal standard adduct, thus giving a noteworthy improvement in the assay precision. The method shows a good reproducibility of the migration times (coefficient of variation, CV%, 1.93) and the peak areas (CV%, 3.65). Intra- and interassay CV were 4.63 and 6.44%, respectively, and analytical recovery was between 98.1 and 102.3%. Assay application was tested measuring taurine plasma levels in 50 healthy volunteers in wh...Continue Reading

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Citations

Jan 31, 2012·Biology of Sex Differences·Ilaria CampesiFlavia Franconi
Sep 14, 2010·Analytical and Bioanalytical Chemistry·Angelo ZinelluCiriaco Carru
Nov 1, 2011·Analytical and Bioanalytical Chemistry·Sanjeewa GamagedaraYinfa Ma
Jul 6, 2010·Current Eye Research·Antonio PinnaCiriaco Carru
Mar 10, 2016·Journal of Diabetes Research·Zaleida NapoliFlavia Franconi
Aug 4, 2015·Journal of Chromatography. a·Raquel Pérez-MíguezMaría Castro-Puyana
Aug 20, 2010·Journal of Pharmaceutical and Biomedical Analysis·Eva Szöko, Tamás Tábi
Jul 4, 2012·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Ilaria CampesiAngelo Zinellu
Dec 19, 2015·European Journal of Clinical Investigation·Angelo ZinelluPietro Pirina
Jun 12, 2014·Luminescence : the Journal of Biological and Chemical Luminescence·M K Sharaf El Din, M E K Wahba
May 8, 2020·European Journal of Clinical Investigation·Angelo ZinelluPietro Pirina

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