Taurocholic acid adsorption during non-starch polysaccharide fermentation: an in vitro study

The British Journal of Nutrition
I C Gelissen, M A Eastwood

Abstract

The association of radiolabelled taurocholic acid with the solid fraction of a faecal fermentation mixture was measured. A human faecal inoculum was incubated with [24-14C]taurocholic acid and several non-starch polysaccharide sources (pectin, wheat bran, ispaghula (Plantago ovata) husk and seed), glucose or a substrate-free control. Portions of fermentation mixture were taken at 0, 3, 6, 21 and 24 h and centrifuged to acquire a supernatant fraction and a pellet containing the fermentation residue. 14C was measured in supernatant fractions and pellets at all time points. Volatile fatty acids (VFA) were measured at 0 and 24 h to confirm bacterial growth. Radioactivity in the pellet increased over time for all substrates. Glucose resulted in the greatest incorporation of taurocholic acid into the pellet, followed by pectin. At 24 h the proportion of the total radioactivity found in the pellet was 92% for glucose, 79% for pectin, 60% for wheat bran, 59% for ispaghula seed, 53% for ispaghula husk and 26% for the control (mean of duplicates). Glucose and pectin produced the greatest quantity of VFA at 24 h. VFA production was highly correlated with radioactivity in the pellet (r0.976, P < 0.005). These results suggest that the bile ...Continue Reading

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Citations

Feb 17, 1996·Lancet·R C Spiller
Jan 11, 2000·QJM : Monthly Journal of the Association of Physicians·M A Eastwood
Sep 1, 2016·Carbohydrate Polymers·Natalia Rosa-SibakovAnna-Marja Aura
Oct 7, 2006·Current Opinion in Gastroenterology·R C Spiller
Jan 1, 2016·Comprehensive Reviews in Food Science and Food Safety·Syed M Nasar-AbbasVijay Jayasena

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