Taurocholic acid is an active promoting factor, not just a biomarker of progression of liver cirrhosis: evidence from a human metabolomic study and in vitro experiments

BMC Gastroenterology
Zhimin LiuZhijun Duan

Abstract

Previous studies have indicated that bile acid is associated with progression of liver cirrhosis. However, the particular role of specific bile acid in the development of liver cirrhosis is not definite. The present study aims to identify the specific bile acid and explore its possible mechanisms in promoting liver cirrhosis. Thirty two cirrhotic patients and 27 healthy volunteers were enrolled. Age, gender, Child-Pugh classification and serum of patients and volunteers were collected. Liquid chromatography tandem mass spectrometry (LC-MS) was utilized to determine concentrations of 12 bile acids in serum. Principal component analysis, fold change analysis and heatmap analysis were used to identify the most changed bile acid. And pathway analysis was used to identify the most affected pathway in bile acid metabolism. Spearman rank correlation analysis was employed to assess correlation between concentrations of bile acids and Child-Pugh classification. Hepatic stellate cells (LX-2) were cultured in DMEM. LX-2 cells were also co-cultured with HepG2 cells in the transwell chambers. LX-2 cells were treated with Na+/taurocholate in different concentrations. Western blot was used to evaluate the expression of alpha smooth muscle act...Continue Reading

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Citations

Jul 12, 2019·Expert Opinion on Drug Metabolism & Toxicology·Yvonne GloorCaroline F Samer
Jun 18, 2019·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Justin D SchumacherGrace L Guo
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Jan 16, 2022·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ana Virseda-BerdicesUNKNOWN ESCORIAL Study Group

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Methods Mentioned

BETA
Protein Assay
PCA
bile
transgenic

Software Mentioned

DA
PLS
MetaboAnalyst
SPSS16

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