Tauroursodeoxycholic acid attenuates gentamicin-induced cochlear hair cell death in vitro

Toxicology Letters
Zhanwei JiaFengyi Li

Abstract

Gentamycin is one of the most clinically used aminoglycoside antibiotics which induce intrinsic apoptosis of hair cells. Tauroursodeoxycholic acid (TUDCA) is known as safe cell-protective agent in disorders associated with apoptosis. We aimed to investigate the protective effects of TUDCA against gentamicin-induced ototoxicity. House Ear Institute-Organ of Corti 1(HEI-OC1) cells and explanted cochlear tissue were treated with gentamicin and TUDCA, followed by serial analyses including cell viability assay, hair cell staining, qPCR, ELISA and western blotting to determine the cell damage by the parameters relevant to cell apoptosis and endoplasmic reticulum stress. TUDCA significantly attenuated gentamicin-induced cell damage in cultured HEI-OC1 cells and explanted cochlear hair cells. TUDCA alleviated gentamicin-induced cell apoptosis, supported by the decreased Bax/Bcl2 ratio compared with that of gentamicin treated alone. TUDCA decreased gentamicin-induced nitric oxide production and protein nitration in both models. In addition, TUDCA suppressed gentamicin-induced endoplasmic reticulum stress as reflected by inversing the expression levels of Binding immunoglobulin protein (Bip), CCAAT/-enhancer-binding protein homologous pr...Continue Reading

Citations

Nov 28, 2019·International Journal of Molecular Sciences·Akihiro KishinoTakeshi Oshima
Jul 10, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu-Hsuan WenHung-Pin Wu
Feb 18, 2020·Neuroscience Letters·Chang Ho LeeSo Young Kim
Jul 3, 2021·Frontiers in Cellular Neuroscience·Xiaolong FuRenjie Chai

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