PMID: 8592097Jan 1, 1996Paper

Tauroursodeoxycholic acid protects in vitro models of human colonic cancer cells from cytotoxic effects of hydrophobic bile acids

The Journal of Laboratory and Clinical Medicine
L L ShekelsS B Ho

Abstract

Bile acids have been implicated as tumor promoters that enhance epithelial proliferation and the development of colonic tumors. This study investigated the effects of bile acids on the growth of in vitro models of human colonic epithelial cells. Cell lines with varying degrees of differentiation (Caco2, HT29, LS174T, and Lovo) were studied. Cell viability and number were measured by a tetrazolium (MTT) spectrophotometric assay. Enhanced cell growth was not observed with any bile acid over the range 10 nmol/L to 2.5 mmol/L. Cytotoxicity was consistently observed at concentrations of unconjugated bile acids greater than 0.1 mmol/L. The bile acid concentration at which 50% growth inhibition occurred was similar for all cell lines and increased in the following order: deoxycholic acid = chenodeoxycholic acid < taurodeoxycholic acid < ursodeoxycholic acid < taurochenodeoxycholic acid < cholic acid < tauroursodeoxycholic acid. Coincubation of tauroursodeoxycholic acid (TUDC) with taurodeoxycholic acid (TDC) or taurochenodeoxycholic acid (TDCD) reversed the short-term (30-minute) cytotoxicity and release of glycoprotein induced by TDC or TCDC regardless of differentiation status. In contrast, TUDC did not reverse the cytotoxicity of d...Continue Reading

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Citations

Feb 1, 1996·The International Journal of Biochemistry & Cell Biology·L L ShekelsS B Ho
Aug 17, 2001·Mutation Research·P R DebruyneM M Mareel
Jan 6, 2001·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Y ArakiT Bamba
Dec 14, 1999·Journal of Applied Microbiology· De Boever P, W Verstraete
Apr 7, 2011·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Yoshiko TakahashiYoshikazu Kinoshita
Jun 15, 2010·Biochemical and Biophysical Research Communications·Yeon Yi LeeKyong Soo Park
May 15, 2008·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·A BozikasJ J G H M Bergman
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Sep 14, 2011·Biochimica Et Biophysica Acta·Josefin Halin, Peter Mattjus
Aug 17, 2005·FEMS Microbiology Reviews·Máire BegleyColin Hill
Jan 20, 2009·Biochimica Et Biophysica Acta·Yong ZhouLenard M Lichtenberger
Dec 12, 2003·World Journal of Gastroenterology : WJG·Zheng WuZuo-Ren Wang
Jun 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Lawrence SerfatyRaoul Poupon
Nov 1, 2018·Journal of Gastroenterology and Hepatology·Young Hoon KimSeong-Joon Koh
Jan 1, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gianfranco AlpiniGene LeSage

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