PMID: 6113842May 26, 1981Paper

Taxol assembles tubulin in the absence of exogenous guanosine 5'-triphosphate or microtubule-associated proteins

Biochemistry
P B Schiff, S B Horwitz

Abstract

Taxol increases the rate and extent of microtubule assembly in vitro and stabilizes microtubules in vitro and in cells [Schiff, P. B., Fant, J., & Horwitz, S. B. (1979) Nature (London) 277, 665-667; Schiff, P. B., & Horwitz, S. B. (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 1561-1565]. We report herein that taxol has the ability to promote microtubule assembly in the absence of microtubule-associated proteins, rings, and added guanosine 5'-triphosphate (GTP or organic buffer. The drug enhances additional microtubule assembly when added to microtubules at apparent steady state. This additional assembly can be attributed to both elongation of existing microtubules and spontaneous nucleation of new microtubules. Taxol-treated microtubules have depressed dissociation reactions as determined by dilution experiments. The drug does not inhibit the binding of GTP or the hydrolysis of GTP or guanosine 5'-diphosphate (GDP) in our microtubule protein preparations. Taxol does not competitively inhibit the binding of colchicine to tubulin.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·I V SandovalP Cuatrecasas
Oct 5, 1977·Journal of Molecular Biology·S M Penningroth, M W Kirschner
Feb 22, 1979·Nature·P B SchiffS B Horwitz
Nov 25, 1977·Journal of Molecular Biology·K A Johnson, G G Borisy
Nov 18, 1975·Biochemistry·J C Lee, S N Timasheff
Oct 28, 1976·Nature·R C Weisenberg, W J Deery
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M D WeingartenM W Kirschner
Nov 15, 1974·Journal of Molecular Biology·F GaskinM L Shelanski
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M L ShelanskiC R Cantor
Sep 29, 1972·Science·G G Borisy, J B Olmsted
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P B Schiff, S B Horwitz

❮ Previous
Next ❯

Citations

Jan 1, 1996·Cell Motility and the Cytoskeleton·C L WymerR J Cyr
Mar 1, 2002·Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions·Martin OberhoffKarl R Karsch
Sep 1, 1990·Journal of Clinical Pharmacology·W J Slichenmyer, D D Von Hoff
Jan 1, 1996·Investigational New Drugs·N L Seibel, G H Reaman
Jan 1, 1986·Cancer Chemotherapy and Pharmacology·J RiondelP Potier
Jan 1, 1993·Cancer Chemotherapy and Pharmacology·N M LopesB K Bhuyan
Oct 1, 1984·Journal of Neurocytology·M RöyttäC S Raine
Aug 1, 1986·Journal of Neurocytology·M Röyttä, C S Raine
Aug 3, 2012·Naunyn-Schmiedeberg's Archives of Pharmacology·Marie EhrlichováIvan Gut
Mar 5, 2008·Cancer Chemotherapy and Pharmacology·Akihiro NakajoUNKNOWN Kyushu Taxol TS-1 Study Group
Oct 8, 2008·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Tsutomu YoshidaHisayoshi Fujiwara
Mar 9, 2005·Breast Cancer Research and Treatment·Kan YonemoriYasuhiro Fujiwara
Dec 17, 2009·Investigational New Drugs·Jana VobořilováJan Kovář
Aug 14, 1996·Chemico-biological Interactions·T PengsuparpJ M Pezzuto
Jan 1, 1983·Toxicon : Official Journal of the International Society on Toxinology·G B BrownL C Tolbert
Jan 1, 1984·Pharmacology & Therapeutics·J J Manfredi, S B Horwitz
Nov 1, 1991·Pharmacology & Therapeutics·J J Correia
Jan 1, 1991·Pharmacology & Therapeutics·L C Morejohn, D E Fosket
Oct 1, 1991·Pharmacology & Therapeutics·E K Rowinsky, R C Donehower
Apr 1, 1992·Trends in Pharmacological Sciences·S B Horwitz
May 12, 1986·Biochimica Et Biophysica Acta·M T Vanier, J F Launay
Jul 9, 1990·Molecular and Cellular Endocrinology·R Ravindra, C E Grosvenor
Feb 15, 1985·Biochimica Et Biophysica Acta·J DeinumP W Jensen
Feb 19, 1986·Biochimica Et Biophysica Acta·M WallinJ Deinum
Jan 1, 1990·Electron Microscopy Reviews·E UngerW Vater
Dec 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M AkutsuY Miura
Aug 28, 2013·Neuropharmacology·Erica Gornstein, Thomas L Schwarz
Jun 1, 1997·Biochemical Pharmacology·C H LieuY K Lai
Mar 7, 2002·International Journal of Pharmaceutics·Anil K SinglaDeepika Aggarwal
Jan 20, 1999·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G N SchwartzP Creaven
Mar 12, 2003·Bioorganic & Medicinal Chemistry·Anastasia NikolakakisLolita O Zamir
Apr 4, 2003·Critical Reviews in Oncology/hematology·Arnaud D Roth
Feb 19, 2000·Journal of Molecular Graphics & Modelling·C B GogoneaS S Siddiqui
Oct 6, 2011·Chemical Reviews·Yu-Fang WangBin Cong

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.