Taxol-producing [corrected] fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata

Journal of Bioscience and Bioengineering
Rangarajulu Senthil KumaranByung-Ki Hur

Abstract

The endophytic fungi, Pestalotiopsis versicolor and Pestalotiopsis neglecta, were isolated from the healthy leaves and bark of the Japanese Yew tree, Taxus cuspidata. The fungal species were identified by their characteristic culture morphology and molecular analysis. For the first time, the test fungi were screened for the production of taxol in modified liquid medium. The presence of taxol was confirmed by HPLC, (1)H NMR, and LC-MS methods of analysis. The maximum amount of taxol production in P. versicolor was recorded as 478 μg/l. The production rate was increased to 9560-fold than that found in the culture broth of earlier reported fungus, Taxomyces andreanae. The extracted fungal taxol showed a strong cytotoxic activity in the in vitro culture of tested human cancer cells by apoptotic assay indicating that the increase in taxol concentration induces increased cell death. A PCR-based screening for ts, a unique gene in the formation of the taxane skeleton, confirmed the molecular blueprint for taxol biosynthesis. The results designate that the fungal endophyte, P. versicolor, is an excellent candidate for an alternate source of taxol supply and can also serve as a potential species for genetic engineering to enhance the pro...Continue Reading

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Citations

May 25, 2012·Applied Microbiology and Biotechnology·Sheela Chandra
Jun 5, 2012·Applied Microbiology and Biotechnology·Ling-Shang WuLu-Ping Qin
Feb 1, 2011·Journal of Bioscience and Bioengineering·Chia-Che ChangYu-Hong Wei
Oct 25, 2014·Critical Reviews in Microbiology·Ling ChenTing Han
Oct 13, 2016·The Journal of Antibiotics·Arnold L Demain, Evan Martens
Jan 18, 2012·Natural Product Reports·Xiao-Long YangDu-Qiang Luo
Jan 27, 2016·Journal of Applied Microbiology·S S El-HawaryM E Rateb
Feb 4, 2021·Journal of Natural Products·Sharna-Kay Daley, Geoffrey A Cordell
Jan 11, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yogeswari SubramaniamJayabaskaran Chelliah
Jun 15, 2021·Bulletin of the National Research Centre·Chijioke E EzeobioraUdoma E Mendie
Jul 1, 2021·Natural Product Research·Taridaporn BunyapaiboonsriSatinee Suetrong

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