PMID: 7541107Dec 1, 1994Paper

Taxonomic classification of 29 Borrelia burgdorferi strains isolated from patients with Lyme borreliosis: a comparison of five different phenotypic and genotypic typing schemes

Medical Microbiology and Immunology
A M LebechM Theisen

Abstract

Twenty-nine European and North American Borrelia burgdorferi strains isolated from patients with Lyme borreliosis, were investigated by restriction fragment length polymorphism (RFLP) of two phylogenetically highly conserved chromosomal genes encoding flagellin (fla) and the p60 common antigen (CA), as well as of the plasmid-borne outer surface protein A (ospA) gene. RFLP of the ospA, fla and CA gene revealed five, two and four distinct subspecies-specific patterns, respectively. RFLP classification of the B. burgdorferi strains was compared with four different classification schemes proposed by others: (i) molecular mass profile of OspA and OspB (Adam et al. [1]); (ii) OspA serotyping (Wilske et al. [34]); (iii) genomic fingerprinting on the central region of the B. burgdorferi fla gene (Picken [24]) and (iv) 16S rRNA signature nucleotide analysis (Marconi and Garon [19]). Results obtained with the different methods correlated highly. All strains classified as B. burgdorferi sensu stricto and B. afzelii could be unequivocally identified as one distinct group by all five typing methods. B. garinii isolates, however, were more heterogeneous and according to RFLP of the CA and ospA gene fell into either two or three subgroups. Th...Continue Reading

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Citations

Jun 20, 2013·International Journal of Medical Sciences·Kerry L ClarkShirley Hartman
Apr 9, 2008·Dermatologic Therapy·Daniela Vanousová, Jana Hercogová
Aug 1, 1997·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Olivier PéterEric Dayer
Nov 8, 2013·Postgraduate Medical Journal·Simon W DubreyWojtek Rakowicz
Sep 7, 2004·Annals of Neurology·Andrew R PachnerDiego Cadavid
Jul 28, 2015·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·R B DessauM Voldstedlund
Jun 1, 1995·Journal of Bacteriology·M TheisenK Hansen

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