Abstract
Ixodes spinipalpis Hadwen & Nuttall and I. neotomae Cooley are enzootic vectors of Borrelia burgdorferi Johnson, Schmid, Hyde, Steigerwalt & Brenner in western North America. The taxa overlap in host preference, habitat, and morphology. Mitochondrial DNA was compared between the taxa to test for reproductive isolation. A 300-bp region of the mitochondrial 16S ribosomal DNA gene was amplified in 28 specimens of I. neotomae and 149 specimens of I. spinipalpis. These products were screened for sequence variation using single-strand conformation polymorphism analysis, and 9 haplotypes were detected. Haplotype frequencies varied between taxa; however, Shannon diversity analysis indicated that most variation arose among collections within each taxon, and no unique haplotypes characterized either one. Phylogenetic analysis of 18 sequences, representing a replicate of each of the 9 haplotypes, was performed with Ixodes pacificus Cooley & Kohls and Ixodes jellisoni Cooley & Kohls as outgroups. Strong monophyletic support was found for a clade containing I. neotomae and I spinipalpis and within this clade no lineages comprised exclusively either taxon. These results argue against treatment of I. neotomae and I. spinipalpis as distinct sp...Continue Reading
Citations
Mar 8, 2014·Parasitology Research·Nina V KulakovaSergei I Belikov
Feb 22, 2014·Molecular and Cellular Probes·Clare A Anstead, Neil B Chilton
Mar 4, 2014·Ticks and Tick-borne Diseases·Janet Foley, Jonah Piovia-Scott
Aug 19, 2014·Ticks and Tick-borne Diseases·Natalia FedorovaRobert S Lane
Oct 1, 2013·Molecular and Cellular Probes·Clare A AnsteadNeil B Chilton
Aug 29, 2003·Journal of Medical Entomology·Lars EisenRobert S Lane
Jun 6, 2009·Journal of Medical Entomology·H R KetchumM T Longnecker
Mar 28, 2001·Journal of Medical Entomology·T J SlowikR M Davis
Dec 29, 2000·Journal of Medical Entomology·S A WrightV L Kramer
Aug 18, 2004·Journal of Medical Entomology·Larisa K VredevoeBradley S Schneider
Jun 21, 2011·Ticks and Tick-borne Diseases·Janet FoleyNathan Nieto
Jun 5, 2007·Molecular and Cellular Probes·Shaun J Dergousoff, Neil B Chilton
May 25, 1999·Journal of Medical Entomology·R S LaneM Hendson
Apr 13, 2007·Journal of Medical Entomology·M T MarrelliT T S Schumaker
Aug 9, 2006·Journal of Medical Entomology·Mario A Rodríguez-PérezThomas R Unnasch
Mar 11, 2004·Systematic Parasitology·Alberto A GuglielmoneJames E Keirans
Feb 1, 2005·Vector Borne and Zoonotic Diseases·Jennifer M AndersonDouglas E Norris
Jun 27, 2006·Vector Borne and Zoonotic Diseases·Scott M ShoneNelson Delgado
Apr 20, 2006·Journal of Medical Entomology·L EisenR S Lane
Oct 11, 2001·American Journal of Veterinary Research·J E FoleyJ E Madigan
Jan 27, 2009·Experimental & Applied Acarology·Alberto A GuglielmoneIvan G Horak
Apr 1, 2008·The Pan-Pacific Entomologist·Joyce E Kleinjan, Robert S Lane
Dec 14, 2019·Experimental & Applied Acarology·F C L ErnieenorA Mariana
Aug 4, 2006·Applied and Environmental Microbiology·Jennifer M Anderson, Douglas E Norris
Sep 17, 1999·Journal of Clinical Microbiology·W L NicholsonJ E Childs
Oct 9, 1999·Clinical Microbiology Reviews·G WangJ Dankert
Nov 18, 1998·Journal of Clinical Microbiology·D PosticG Baranton
Oct 23, 2009·Journal of Clinical Microbiology·Nataliia RudenkoJames H Oliver