Taylor Dispersion Analysis of Polysaccharides Using Backscattering Interferometry

Analytical Chemistry
Phoonthawee SaetearHervé Cottet

Abstract

Taylor dispersion analysis (TDA) allows the determination of the molecular diffusion coefficient (D) or the hydrodynamic radius (Rh) of a solute from the peak broadening of a plug of solute in a laminar Poiseuille flow. The main limitation plaguing the broader applicability of TDA is the lack of a sensitive detection modality. UV absorption is typically used with TDA but is only suitable for UV-absorbing or derivatized compounds. In this work, we present a development of the TDA method for non-UV absorbing compounds by using a universal detector based on refractive index (RI) sensing with backscattering interferometry (BSI). BSI was interfaced to a capillary electrophoresis-UV instrument using a polyimide coated fused silica capillary and an in-house designed flow-cell assembly. Polysaccharides were selected to demonstrate the application of TDA-BSI for size characterization. Under the conditions of validity of TDA, D and Rh average values and the entire Rh distributions were obtained from the (poly)saccharide taylorgrams, including non-UV absorbing polymers.

References

Jul 25, 2000·Analytical Chemistry·K SwinneyD J Bornhop
Oct 26, 2007·Analytical Chemistry·Hervé CottetMichel Martin
Jan 11, 2008·Journal of Agricultural and Food Chemistry·Marshall L FishmanArland T Hotchkiss
Mar 29, 2011·International Journal of Pharmaceutics·Wendy L Hulse, Robert T Forbes
Nov 9, 2011·Analytical Chemistry·Amanda KussrowDarryl J Bornhop
Mar 19, 2013·Analytical Chemistry·Anna LewandrowskaRobert Hołyst
Dec 4, 2015·Biomacromolecules·Joseph ChamiehHervé Cottet
Mar 11, 2016·Proceedings of the National Academy of Sciences of the United States of America·Darryl J BornhopJens Meiler
Jul 28, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Ulrich B HøgstedtJesper Østergaard

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