TB tools to tell the tale-molecular genetic methods for mycobacterial research

The International Journal of Biochemistry & Cell Biology
Edith E MachowskiValerie Mizrahi

Abstract

In spite of the availability of drugs and a vaccine, tuberculosis--one of man's medical nemeses--remains a formidable public health problem, particularly in the developing world. The persistent nature of the tubercle bacillus, with one third of the world's population is estimated to be infected, combined with the emergence of multi drug-resistant strains and the exquisite susceptibility of HIV-positive individuals, has underscored the urgent need for in-depth study of the biology of Mycobacterium tuberculosis address the resurgence of TB. In aiming to understand the mechanisms by which mycobacteria react to their immediate environments, molecular genetic tools have been developed from naturally occurring genetic elements. These include protein expressing genes, and episomal and integrating elements, which have been derived mainly from prokaryotic but also from eukaryotic organisms. Molecular genetic tools that had been established as routine procedures in other prokaryotic genera were thus mimicked. Knowledge of the underlying mechanisms greatly expedited the harnessing of these elements for mycobacteriological research and has brought us to a point where these molecular genetic tools are now employed routinely in laboratories ...Continue Reading

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Citations

Jul 19, 2012·Yi chuan = Hereditas·Qin-Lin DuJian-Ping Xie
Mar 4, 2008·Microbiology·Michael Niederweis
Jul 24, 2010·Journal of Microbiological Methods·Ricardo BalhanaSharon L Kendall
Feb 9, 2017·Memórias do Instituto Oswaldo Cruz·Caroline RizziOdir Antônio Dellagostin
Mar 6, 2012·Risk Analysis : an Official Publication of the Society for Risk Analysis·Elisabeth Paté-Cornell

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