TBC1D5-Catalyzed Cycling of Rab7 Is Required for Retromer-Mediated Human Papillomavirus Trafficking during Virus Entry.

Cell Reports
Jian XieDaniel DiMaio

Abstract

During virus entry, human papillomaviruses are sorted by the cellular trafficking complex, called retromer, into the retrograde transport pathway to traffic from the endosome to downstream cellular compartments, but regulation of retromer activity during HPV entry is poorly understood. Here we selected artificial proteins that modulate cellular proteins required for HPV infection and discovered that entry requires TBC1D5, a retromer-associated, Rab7-specific GTPase-activating protein. Binding of retromer to the HPV L2 capsid protein recruits TBC1D5 to retromer at the endosome membrane, which then stimulates hydrolysis of Rab7-GTP to drive retromer disassembly from HPV and delivery of HPV to the retrograde pathway. Although the cellular retromer cargos CIMPR and DMT1-II require only GTP-bound Rab7 for trafficking, HPV trafficking requires cycling between GTP- and GDP-bound Rab7. Thus, ongoing cargo-induced membrane recruitment, assembly, and disassembly of retromer complexes drive HPV trafficking.

References

Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·E C GoodwinE S Hwang
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·E C Goodwin, D DiMaio
Oct 11, 2001·The Journal of Biological Chemistry·E SzaferD Cassel
Apr 14, 2004·The Journal of Cell Biology·Matthew N J Seaman
Feb 15, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Maria Rita SpinosaCecilia Bucci
Oct 28, 2008·Computer Methods and Programs in Biomedicine·A Allalou, C Wählby
Nov 5, 2008·The Journal of Cell Biology·Raul RojasJuan S Bonifacino
Apr 7, 2009·Methods in Molecular Biology·Jim SunZakaria Hmama
Feb 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tobin J CammettDaniel Dimaio
Dec 14, 2011·Traffic·Martina Bergant MarušičLawrence Banks
May 18, 2012·Molecular Biology of the Cell·Ting-Ting LiuChristopher G Burd
Jun 8, 2012·Neurobiology of Aging·Badri N VardarajanLindsay A Farrer
Nov 14, 2012·Journal of Cell Science·Matthew N J Seaman
Apr 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Alex LipovskyDaniel DiMaio
Dec 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Megan S HarrisonChristopher G Burd
Feb 5, 2014·Cold Spring Harbor Perspectives in Biology·Christopher Burd, Peter J Cullen
Aug 12, 2015·Proceedings of the National Academy of Sciences of the United States of America·Erin N HeimDaniel DiMaio
May 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Stephen DiGiuseppeMartin J Sapp
Aug 23, 2016·Cells·Flora Guerra, Cecilia Bucci
Nov 9, 2016·Nature Communications·Da JiaMichael K Rosen
Nov 28, 2016·Cell·María LucasAitor Hierro
Dec 13, 2017·Proceedings of the National Academy of Sciences of the United States of America·Miguel Romano-MorenoAitor Hierro
Jan 13, 2018·Journal of Virology·Abida SiddiqaLawrence Banks
Feb 2, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jialin YaoDa Jia

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Citations

Feb 3, 2021·Trends in Biochemical Sciences·Matthew N J Seaman
Mar 23, 2021·Frontiers in Cell and Developmental Biology·Xin YongDa Jia
Mar 19, 2021·Journal of Virology·Marina Bugnon ValdanoLawrence Banks
Aug 21, 2021·Current Opinion in Virology·Michelle A Ozbun, Samuel K Campos
Sep 3, 2021·Journal of Molecular Biology·Lisa M PettiDaniel DiMaio

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Methods Mentioned

BETA
GTPase
immunoprecipitation
proximity ligation
pull-downs
electrophoresis
pull-down
co-immunoprecipitation
flow cytometry
confocal microscopy
transfectability

Software Mentioned

BlobFinder
GraphPad Prism
ImageJ
Fiji
Imaris

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