TbMP81 is required for RNA editing in Trypanosoma brucei

The EMBO Journal
M DrozdzKenneth Stuart

Abstract

Most mitochondrial mRNAs are edited in Trypano soma brucei by a series of steps that are catalyzed by a multienzyme complex that is in its initial stages of characterization. RNA interference (RNAi)-mediated repression of the expression of TbMP81, a zinc finger protein component of the complex, inhibited growth of bloodstream and insect forms, and blocked in vivo RNA editing. This repression preferentially inhibited insertion editing compared with deletion editing in vitro. It resulted in reduced specific endoribonucleolytic cleavage and a greater reduction of U addition and associated RNA ligation activities than U removal and associated RNA ligation activities. The repressed cells retained 20S editing complexes with several demonstrable proteins and adenylatable TbMP52 RNA ligase, but adenlyatable TbMP48 was not detected. Elimination of TbMP48 by RNAi repression did not inhibit cell growth or in vivo editing in either bloodstream or procyclic forms. These results indicate that TbMP81 is required for RNA editing and suggest that the editing complex is functionally partitioned.

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Feb 19, 2002·Molecular and Biochemical Parasitology·Reza SalavatiKenneth Stuart

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Citations

May 28, 2009·Journal of Molecular Modeling·Alireza Shaneh, Reza Salavati
Apr 4, 2003·Molecular and Biochemical Parasitology·Setareh S PalazzoKenneth Stuart
Jul 3, 2003·Journal of the American Society for Mass Spectrometry·Aswini K PanigrahiSteven P Gygi
Jul 26, 2002·Molecular Microbiology·Kenneth Stuart, Aswini K Panigrahi
Nov 7, 2003·Nucleic Acids Research·Elizabeth A WortheyReza Salavati
May 7, 2010·Nucleic Acids Research·Houtan Moshiri, Reza Salavati
Jun 11, 2003·Eukaryotic Cell·Gonzalo J DomingoKenneth D Stuart
Jul 22, 2009·Molecular and Cellular Biology·John C FiskLaurie K Read
Mar 16, 2005·Molecular and Cellular Biology·Julie A LawBarbara Sollner-Webb
May 23, 2007·Molekuliarnaia biologiia·R Afasizhev
Jun 20, 2006·BMC Bioinformatics·I Saira MianReza Salavati
Jul 11, 2006·BMC Biotechnology·Martin C Taylor, John M Kelly
Mar 26, 2009·RNA·Nancy Lewis ErnstKenneth Stuart
Mar 22, 2003·RNA·Aswini K PanigrahiKenneth Stuart
Feb 24, 2006·RNA·Alevtina G ZhelonkinaBarbara Sollner-Webb
Apr 14, 2006·RNA·Aswini K PanigrahiKenneth D Stuart
Sep 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ruslan AphasizhevLarry Simpson
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jonatha M Gott
Apr 1, 2010·Expert Opinion on Drug Discovery·Robert V Swift, Rommie E Amaro
Nov 18, 2015·Trends in Parasitology·Inna Aphasizheva, Ruslan Aphasizhev
Apr 7, 2010·Journal of Molecular Biology·Gene-Errol RingpisRuslan Aphasizhev
Aug 1, 2015·FEBS Letters·Anna CzerwoniecJanusz M Bujnicki
Dec 26, 2006·Experimental Parasitology·Tracy SaveriaMarilyn Parsons
Oct 29, 2003·Trends in Parasitology·Alan H Fairlamb
Aug 9, 2011·Wiley Interdisciplinary Reviews. RNA·Ruslan Aphasizhev, Inna Aphasizheva
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Anne-Laure Chateigner-Boutin, Ian Small
Feb 5, 2005·Trends in Biochemical Sciences·Kenneth D StuartAswini K Panigrahi

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African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.