TbRAB1 and TbRAB2 mediate trafficking through the early secretory pathway of Trypanosoma brucei

Molecular and Biochemical Parasitology
Vivek DhirMark C Field

Abstract

The African trypanosome possesses a total of 16 small GTPases of the Rab family, which are involved in control of various membrane transport events. Recently the roles of these proteins in the endocytosis and recycling of the major surface antigen of the bloodstream form, the variant surface glycoprotein (VSG), have been described but little has been reported on the roles of Rab proteins in exocytic pathways in trypanosomatids. Whilst phylogenetic analysis based on sequence similarity indicates a comparatively well conserved core set of Rab proteins, the evolutionary distance of the trypanosome lineage from crown eukaryote model systems requires direct experimental evidence to support these sequence data. By database searching we identified two further Rab genes, TbRAB1 and TbRAB2, which are the trypanosome sequence orthologues of mammalian Rab1 and Rab2, important mediators of ER to Golgi and intra-Golgi transport processes. A remarkably high level of sequence conservation is retained between the trypanosome and higher eukaryote orthologues. By immunolocalisation we find that both TbRAB1 and TbRAB2 reside on membranes in intimate association with the Golgi complex. By heterologous expression in mammalian cells we also demonstr...Continue Reading

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Citations

Jun 29, 2010·Cellular and Molecular Life Sciences : CMLS·Andrew BrighouseMark C Field
Apr 7, 2005·Molecular and Biochemical Parasitology·John P AckersMark C Field
Oct 7, 2009·Nature Reviews. Microbiology·Mark C Field, Mark Carrington
Mar 15, 2013·Molecular Biology of the Cell·Carme Gabernet-CastelloMark C Field
Jun 7, 2011·Eukaryotic Cell·Carme Gabernet-CastelloMark C Field
May 10, 2005·Eukaryotic Cell·Belinda S HallMark C Field
Oct 10, 2006·BMC Genomics·Sébastien BesteiroJeremy C Mottram
Nov 22, 2011·Journal of Cell Science·Jennifer H LumbMark C Field
Sep 18, 2007·Molecular and Biochemical Parasitology·Clare L AllenMark C Field
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Jan 17, 2007·Cellular Microbiology·Cynthia Y He
Apr 28, 2007·Traffic·Mark C FieldV Lila Koumandou
Feb 12, 2009·Traffic·Jordan T YelinekGraham Warren
Jan 25, 2006·Biochemical and Biophysical Research Communications·Mehdi ChenikKoussay Dellagi
Nov 26, 2013·Parasitology International·Edwin MurungiAlan Christoffels
Nov 4, 2004·Traffic·Mark C Field, Mark Carrington
Oct 27, 2015·The Journal of Biological Chemistry·Surbhi BahlAmitabha Mukhopadhyay
Apr 16, 2008·Journal of Cell Science·Irene Barinaga-Rementeria RamirezGraham Warren
Sep 14, 2014·Journal of Cell Science·Anaïs BrasseurCynthia Y He
Aug 24, 2021·Frontiers in Cell and Developmental Biology·Fabian LinkMarkus Engstler

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African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.