TCDD-induced multi- and transgenerational changes in the methylome of male zebrafish gonads.

Environmental Epigenetics
Camille AkemannTracie R Baker

Abstract

The legacy endocrine disrupting chemical and aryl hydrocarbon receptor agonist, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is produced as a byproduct of industrial processes and causes adverse health effects ranging from skin irritation to cancer. TCDD endpoints are also observed in subsequent, unexposed generations; however, the mechanisms of these multi- and transgenerational effects are unknown. We hypothesized an epigenetic mechanism, specifically DNA methylation for the transgenerational, male-mediated reproductive effects of developmental TCDD exposure. Using whole genome bisulfite sequencing, we evaluated DNA methylation changes in three generations of zebrafish, the first of which was exposed to TCDD during sexual development at 50 ppt for 1 h at both 3- and 7-week post-fertilization. We discovered that TCDD induces multi- and transgenerational methylomic changes in testicular tissue from zebrafish with decreased reproductive capacity, but most significantly in the indirectly exposed F1 generation. In comparing differentially methylated genes to concurrent transcriptomic changes, we identified several genes and pathways through which transgenerational effects of low level TCDD exposure are likely inherited. These inclu...Continue Reading

References

Jan 14, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·J MimuraY Fujii-Kuriyama
Jun 24, 2000·Lancet·P MocarelliL L Needham
Oct 6, 2000·Andrologia·S SignoriniP Mocarelli
Aug 29, 2001·The Journal of Biological Chemistry·N Cervoni, M Szyf
Oct 26, 2001·The Journal of Biological Chemistry·Hung-Ying KaoRonald M Evans
Sep 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Nevan J KroganJack F Greenblatt
May 19, 2006·Cancer Research·Allen S YangJean-Pierre J Issa
May 19, 2006·The Journal of Biological Chemistry·Shannon M Lauberth, Michael Rauchman
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ana C D'Alessio, Moshe Szyf
Sep 22, 2006·The Biochemical Journal·Shivakumara BheemanaikDesirazu N Rao
Nov 29, 2007·Genomics·Matthew D AnwayMichael K Skinner
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Jun 22, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nancy J NesslingerBrad H Nelson
Nov 11, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Christelle Stouder, Ariane Paoloni-Giacobino
Apr 16, 2011·Bioinformatics·Felix Krueger, Simon R Andrews
Jun 11, 2011·Neuron·Jeremy J Day, J David Sweatt
Jul 13, 2011·Molecular and Cellular Biology·Darah A ChristieJoaquín Madrenas
Jul 3, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Tracie R BakerWarren Heideman
Jul 17, 2013·Particle and Fibre Toxicology·Hyang-Min ByunAndrea A Baccarelli
Jan 29, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Tracie R BakerWarren Heideman
Sep 10, 2014·Molecular and Cellular Endocrinology·Tracie R BakerWarren Heideman
Oct 7, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Joshua A HarrillRussell S Thomas
Jan 9, 2015·The Journal of Experimental Biology·Warren W Burggren
Sep 2, 2016·Occupational and Environmental Medicine·Andrea 't MannetjeNeil Pearce

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Citations

Apr 27, 2021·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·Harrison D Wang, Patrick Allard

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Datasets Mentioned

BETA
GSE149919

Methods Mentioned

BETA
dissection

Software Mentioned

bowtie2
Transcriptome Analysis Console ( TAC
bismark
IPA
methylKit

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