PMID: 11342101May 9, 2001Paper

TCF11/Nrf1 overexpression increases the intracellular glutathione level and can transactivate the gamma-glutamylcysteine synthetase (GCS) heavy subunit promoter

Biochimica Et Biophysica Acta
M C MyhrstadA Kolstø

Abstract

Gamma-glutamylcysteinylglycine or glutathione (GSH) performs important protective functions in the cell through maintenance of the intracellular redox balance and elimination of xenobiotics and free radicals. The production of GSH involves a number of enzymes and enzyme subunits offering multiple opportunities for regulation. Two members of the CNC subfamily of bZIP transcription factors (TCF11/Nrf1 and Nrf2) have been implicated in the regulation of detoxification enzymes and the oxidative stress response. Here we investigate the potential role of one of these factors, TCF11/Nrf1, in the regulation of GSH levels in the cell and particularly its influence on the expression of one of the enzymatic components necessary for the synthesis of GSH, the heavy subunit of gamma-glutamylcysteine synthetase (GCS(h)). Using overexpression of the transcription factor in COS-1 cells we show that TCF11/Nrf1 stimulates GSH accumulation. Using co-transfection with reporter constructs where reporter expression is driven through the GCS(h) promoter we show that this increase may be mediated in part by induced expression of the GCS(h) gene by TCF11/Nrf1. We further show that a distal portion of the promoter including two antioxidant-response eleme...Continue Reading

References

Apr 16, 1996·Biochemical and Biophysical Research Communications·V NatarajanJ O Moskaug
Nov 1, 1996·Nucleic Acids Research·O JohnsenA B Kolstø
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·R Venugopal, A K Jaiswal
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·W W Wasserman, W E Fahl
Jul 29, 1998·Chemico-biological Interactions·M E Anderson
Feb 26, 1999·The Journal of Biological Chemistry·A KobayashiM Yamamoto
Nov 24, 1999·The Journal of Biological Chemistry·A C WildR T Mulcahy
Nov 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Steven W ColeStephen J Suomi

❮ Previous
Next ❯

Citations

Jun 25, 2002·Molecular Aspects of Medicine·Malcolm J JacksonSiân B Astley
Oct 7, 2009·Journal of Environmental Sciences (China)·Cailin GeShishi Luo
Jun 17, 2003·Molecular and Cellular Biology·Linyun ChenJefferson Y Chan
Oct 3, 2002·Annual Review of Pharmacology and Toxicology·Truyen NguyenCecil B Pickett
Jun 14, 2003·Redox Report : Communications in Free Radical Research·Nicole Elizabeth Sparling, Shelley Ann Phelan
Jan 20, 2016·International Journal of Molecular Sciences·Janina DoseTuba Esatbeyoglu
Feb 1, 2015·FEBS Letters·Daniel V Ho, Jefferson Y Chan
Apr 17, 2012·Seminars in Cell & Developmental Biology·Hongqiao Zhang, Henry Jay Forman
Aug 12, 2009·Toxicology and Applied Pharmacology·Madhurima Biswas, Jefferson Y Chan
Jul 8, 2008·Molecular Aspects of Medicine·Shelly C Lu
Nov 23, 2006·Brain Research·Alejandra M PacchioniPeter W Kalivas
Jun 20, 2006·Biochemical and Biophysical Research Communications·Eva Hernandez-MontesJeremy P E Spencer
Sep 22, 2012·Biochimica Et Biophysica Acta·Shelly C Lu
May 6, 2003·Biochimica Et Biophysica Acta·Cathrine HusbergAnne-Brit Kolstø
Feb 21, 2006·Antioxidants & Redox Signaling·Kazuhiko Igarashi, Jiying Sun
Jun 20, 2017·Free Radical Biology & Medicine·Jeong Woo HanJefferson Y Chan
Feb 15, 2011·Antioxidants & Redox Signaling·Franziska KriegenburgRasmus Hartmann-Petersen
Jan 7, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dale A DickinsonHenry Jay Forman
Jul 30, 2002·The Journal of Biological Chemistry·Laurie M Zipper, R Timothy Mulcahy
Jul 5, 2007·The Journal of Biological Chemistry·Weiping WangJefferson Y Chan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.