TCF7L2 regulates postmitotic differentiation programmes and excitability patterns in the thalamus.

Development
M. A. LipiecMarta Barbara Wisniewska

Abstract

Neuronal phenotypes are controlled by terminal selector transcription factors in invertebrates, but only a few examples of such regulators have been provided in vertebrates. We hypothesised that TCF7L2 regulates different stages of postmitotic differentiation in the thalamus, and functions as a thalamic terminal selector. To investigate this hypothesis, we used complete and conditional knockouts of Tcf7l2 in mice. The connectivity and clustering of neurons were disrupted in the thalamo-habenular region in Tcf7l2-/- embryos. The expression of subregional thalamic and habenular transcription factors was lost and region-specific cell migration and axon guidance genes were downregulated. In mice with a postnatal Tcf7l2 knockout, the induction of genes that confer thalamic terminal electrophysiological features was impaired. Many of these genes proved to be direct targets of TCF7L2. The role of TCF7L2 in terminal selection was functionally confirmed by impaired firing modes in thalamic neurons in the mutant mice. These data corroborate the existence of master regulators in the vertebrate brain that control stage-specific genetic programmes and regional subroutines, maintain regional transcriptional network during embryonic developme...Continue Reading

Associated Datasets

Jul 24, 2020·Marcin LipiecMarta B Wiśniewska

References

Nov 1, 1995·Mechanisms of Development·N G Fedtsova, E E Turner
Aug 7, 1999·Science·E M Miyashita-LinJ L Rubenstein
Aug 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Bin ChenSusan K McConnell
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Nov 14, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Okihide HikosakaPaul D Shepard
Mar 17, 2009·Nature·Nuria Flames, Oliver Hobert
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Nov 13, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lely A QuinaEric E Turner
Dec 18, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Banafsheh KadkhodaeiThomas Perlmann
Apr 2, 2010·BMC Bioinformatics·Robert C McLeay, Timothy L Bailey
Apr 8, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Marta B WisniewskaJacek Kuznicki
May 4, 2010·Nature Neuroscience·Tomomi ShimogoriSeth Blackshaw
Jun 19, 2010·Nature Reviews. Neuroscience·Okihide Hikosaka
Sep 8, 2010·Nature Neuroscience·Chen LiuEvan S Deneris
Dec 31, 2010·The Journal of Comparative Neurology·Kazuya YugeTomomi Shimogori
May 6, 2011·Bioinformatics·Timothy L Bailey
May 23, 2012·Nucleic Acids Research·Timothy L Bailey, Philip Machanick
Oct 2, 2012·Cold Spring Harbor Perspectives in Biology·Ken M Cadigan, Marian L Waterman
Nov 9, 2012·Development·Mallika ChatterjeeJames Y H Li
Nov 24, 2012·Nucleic Acids Research·Ivan V KulakovskiyVsevolod J Makeev
Sep 17, 2013·Gene Expression Patterns : GEP·Kathryn M LehighMaria J Donoghue
Sep 27, 2013·Neural Development·Carlo A BerettaMatthias Carl
May 24, 2014·Nature Protocols·Wenxiu MaTimothy L Bailey
Sep 10, 2014·Current Biology : CB·Ulrike HüskenMatthias Carl
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber

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Citations

Mar 7, 2021·Biomolecules·Anja Bühler, Matthias Carl
Apr 21, 2021·Nature Biotechnology·Halima Hannah SchedeGioele La Manno

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Methods Mentioned

BETA
RNA-seq
ChIP-seq
ChIP
sedation
protein assay
PCR

Software Mentioned

EdgeR
pClamp10
BWA
DeSeq2
HISAT
GOrilla
MEME
ImageJ
ChIPseeker
HTSeq

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