TCP Transcription Factors Interact With NPR1 and Contribute Redundantly to Systemic Acquired Resistance

Frontiers in Plant Science
Min LiZheng Qing Fu

Abstract

In Arabidopsis, TEOSINTE BRANCHED 1, CYCLOIDEA, PCF1 (TCP) transcription factors (TF) play critical functions in developmental processes. Recent studies suggest they also function in plant immunity, but whether they play an important role in systemic acquired resistance (SAR) is still unknown. NON-EXPRESSER OF PR GENES 1 (NPR1), as an essential transcriptional regulatory node in SAR, exerts its regulatory role in downstream genes expression through interaction with TFs. In this work, we provide biochemical and genetic evidence that TCP8, TCP14, and TCP15 are involved in the SAR signaling pathway. TCP8, TCP14, and TCP15 physically interacted with NPR1 in yeast two-hybrid assays, and these interactions were further confirmed in vivo. SAR against the infection of virulent strain Pseudomonas syringae pv. maculicola (Psm) ES4326 in the triple T-DNA insertion mutant tcp8-1 tcp14-5 tcp15-3 was partially compromised compared with Columbia 0 (Col-0) wild type plants. The induction of SAR marker genes PR1, PR2, and PR5 in local and systemic leaves was dramatically decreased in the tcp8-1 tcp14-5 tcp15-3 mutant compared with that in Col-0 after local treatment with Psm ES4326 carrying avrRpt2. Results from yeast one-hybrid and chromatin i...Continue Reading

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Citations

Sep 17, 2020·Plant Molecular Biology·Lucia V FerreroDaniel H Gonzalez
Jul 11, 2019·International Journal of Molecular Sciences·Ian Arthur PalmerZheng Qing Fu
Jan 27, 2021·Plant, Cell & Environment·Jian ChenFengquan Liu
Sep 29, 2019·Plant Phenomics : a Science Partner Journal·Steven YatesBruce A McDonald
Jan 31, 2021·International Journal of Molecular Sciences·Jamie A O'Rourke, Michelle A Graham
Dec 30, 2020·International Journal of Molecular Sciences·Edoardo SalladiniKaren Skriver
Mar 7, 2021·Trends in Plant Science·Evi CeulemansAlain Goossens
Dec 4, 2021·Frontiers in Plant Science·Qingfeng ZhouHang Gao

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Methods Mentioned

BETA
transgenic
PCR
two-hybrid
one-hybrid
ChIP
Y2H
Co-immunoprecipitation
Co-IP
immunoprecipitation
Y1H

Software Mentioned

Excel
GraphPad Prism

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