PMID: 9435261Jan 22, 1998Paper

TcpP protein is a positive regulator of virulence gene expression in Vibrio cholerae

Proceedings of the National Academy of Sciences of the United States of America
C C Häse, J J Mekalanos

Abstract

The production of several virulence factors in Vibrio cholerae O1, including cholera toxin and the pilus colonization factor TCP (toxin-coregulated pilus), is strongly influenced by environmental conditions. To specifically identify membrane proteins involved in these signal transduction events, we examined a transposon library of V. cholerae generated by Tnbla mutagenesis for cells that produce TCP when grown under various nonpermissive conditions. To select for TCP-producing cells we used the recently described bacteriophage CTX phi-Kan, which uses TCP as its receptor and carries a gene encoding resistance to kanamycin. Among the isolated mutants was a transposon insertion in a gene homologous to nqrB from Vibrio alginolyticus, which encodes a subunit of a Na(+)-translocating NADH:ubiquinone oxidoreductase, and tcpI, encoding a chemo-receptor previously implicated in the negative regulation of TCP production. A third transposon mutant had an insertion in tcpP, which is in an operon with tcpH, a known positive regulator of TCP production. However, TcpP was shown to be essential for TCP production in V. cholerae, as a tcpP-deletion strain was deficient in pili production. The amino-terminal region of TcpP shows sequence homolog...Continue Reading

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Citations

Jun 19, 2002·FEMS Microbiology Reviews·Joachim Reidl, Karl E Klose
Dec 21, 2000·Trends in Microbiology·C C Häse
May 7, 2003·Current Opinion in Microbiology·Eric S Krukonis, Victor J DiRita
Jun 7, 2002·Nature·D Scott MerrellAndrew Camilli
Apr 16, 2002·Molecular Microbiology·Caiyi C LiJames B Kaper
Nov 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jun ZhengJohn J Mekalanos
Sep 29, 2012·The Journal of Biological Chemistry·Kang-Mu LeeSang Sun Yoon
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D S Merrell, A Camilli
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·V J DiRitaR Yu
Oct 11, 2001·Microbiology and Immunology·K BiS Shinoda
Apr 10, 2002·Microbiology and Immunology·Shah M Faruque, G Balakrish Nair
Jul 18, 2002·Antimicrobial Agents and Chemotherapy·Pavel DibrovClaudia C Häse
May 26, 2001·Applied and Environmental Microbiology·I N RiveraR R Colwell
Jul 1, 2009·Infection and Immunity·Basel H Abuaita, Jeffrey H Withey
Sep 19, 2007·Infection and Immunity·Jyl S MatsonVictor J DiRita
May 23, 2006·Infection and Immunity·Sinem BeyhanFitnat H Yildiz
Feb 17, 2001·Infection and Immunity·D K KaraolisP R Reeves
May 12, 2001·Infection and Immunity·M Sandkvist
Dec 19, 2001·Infection and Immunity·Jamie A WibbenmeyerAnne H Delcour
Sep 23, 2003·Infection and Immunity·Nilanjan SenguptaRukhsana Chowdhury
Jan 27, 2004·Infection and Immunity·Hua XieRichard J Lamont
May 25, 2004·Infection and Immunity·Jyoti Mathur, Matthew K Waldor
Jun 24, 2004·Infection and Immunity·H H KrishnanRukhsana Chowdhury

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