PMID: 9438200Jan 23, 1998Paper

TCR expression and clonality analysis in peripheral blood and lymph nodes of HIV-infected patients

Human Immunology
M MionA Amadori

Abstract

We compared the T cell receptor (TCR) V beta gene family repertoire in peripheral blood mononuclear cells (PBMC) and lymph node (LN) cells from 7 human immunodeficiency virus (HIV)-infected patients and 3 seronegative healthy controls. Virtually all the V beta family specificities were represented in patient PBMC and LN cells, and mean values for each specificity were comparable to figures in seronegative controls. In 4 patients, however, some V beta gene segment transcripts were overrepresented in the LN compartment, compared to the peripheral blood counterpart. To ascertain whether this phenomenon was due to polyclonal or oligoclonal expansion of T cells bearing the relevant V beta gene product, we sequenced the entire CDR3 region of a panel of 238 PCR clones corresponding to the V beta transcripts expanded in LN; as control, the same regions were cloned and sequenced in patient's PBMC, and in PBMC and LN cells from seronegative individuals. This analysis disclosed preferential usage of J beta 2 genes in PBMC and LN cells from both seropositive patients and controls, regardless of the V beta gene segment considered, thus indicating that this skewness in the V beta-J beta repertoire could be a consistent feature of at least a ...Continue Reading

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Citations

Apr 12, 2005·Journal of Clinical Immunology·Carmem Beatriz Wagner Giacoia-GrippMariza Gonçalves Morgado
Mar 28, 2002·Human Immunology·Frank M RaaphorstJudy M Teale
Nov 6, 2002·The Journal of Clinical Investigation·Sang Kyung LeePremlata Shankar
Jul 8, 2009·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·D SalameireM C Jacob
Oct 16, 2010·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Lenka TesarovaMartin Klabusay
Sep 26, 2002·Clinical and Experimental Immunology·M D Bodman-SmithA Zumla
Jul 17, 1998·Clinical and Diagnostic Laboratory Immunology·S N Liossis, G C Tsokos

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