TCRB junctional regions from HLA-B27-restricted T cells and HLA-B27 binding peptides display conserved hydropathy profiles in the absence of primary sequence homology

International Immunology
E MayE Märker-Hermann

Abstract

Analysis of formal amino acid sequence identity between different TCRB chain (TCRB) hypervariable regions (CDR3) is commonly used to localize relevant sites of TCR antigen interaction or to yield indirect information on unknown corresponding antigens. However, this analysis sometimes fails to demonstrate expected concordances, e.g. between CDR3 from T cell clones of identical reactivity. Since this may be due to ignorance of physico-chemical parameters, we have now use hydropathy profile analysis as an additional method to examine TCRB-CDR3 and putative peptide antigens. Superimposed hydropathy plots (SHOP) of 20 TCRB-CDR3 from HLA-B27-restricted autoreactive and Yersinia enterocolitica-specific synovial cytotoxic T lymphocytes (CTL) isolated from patients with reactive arthritis (ReA) revealed restricted distribution of polar amino acids resulting in characteristically different SHOP profiles between the two CTL groups. Similarly, Yersinia-derived and self nonapeptides known to bind HLA-B27 differed in SHOP profiles. To validate the method we have extended SHOP analysis to published TCRB sequence data from additional TCRB-CDR3 from peptide-specific CTL but not in TCRB from HLA-B27-alloreactive CTL or non-HLA-B27-restricted con...Continue Reading

Citations

Apr 2, 1998·Current Opinion in Immunology·J A López de Castro
Jul 20, 1999·Current Opinion in Rheumatology·S GonzálezC López-Larrea
Jan 19, 1999·Rheumatic Diseases Clinics of North America·E Märker-Hermann, T Höhler
Apr 15, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R Friedl-HajekR J Joost van Neerven
Jul 18, 2002·The Journal of Biological Chemistry·Manuel RamosJosé A López de Castro
Jul 29, 1997·International Journal of Cancer. Journal International Du Cancer·M HeikeK H Meyer zum Büschenfelde

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