TcRho1 of Trypanosoma cruzi: role in metacyclogenesis and cellular localization

Biochemical and Biophysical Research Communications
Luiz Dione Barbosa de MeloUlisses Gazos Lopes

Abstract

Here we have investigated the function of TcRho1, a Rho family orthologue from the parasite Trypanosoma cruzi. We have selected parasites overexpressing wild-type TcRho1 and a truncated form of TcRho1 (TcRho1-DeltaCaaX) which is unable to undergo farnesylation and supposed to interfere with recruitment of Rho effectors to membranes. TcRho1 protein was localized at the anterior region of wild-type and TcRho1 overexpressing epimastigotes, suggesting association with the Golgi apparatus. Accordingly, parasites overexpressing TcRho1-DeltaCaaX presented cytoplasmic fluorescence. To address the function of TcRho1 during differentiation, from epimastigotes to trypomastigotes, we submitted parasites overexpressing the above-cited lineages to metacyclogenesis assays. Parasites overexpressing TcRho1-DeltaCaaX generated a discrete number of metacyclic trypomastigotes when compared with other lineages. Strikingly, TcRho1-DeltaCaaX cells died synchronously during the process of metacyclogenesis.

References

Sep 1, 1985·Molecular and Biochemical Parasitology·V T ContrerasS Goldenberg
Nov 1, 1993·Molecular and Biochemical Parasitology·R CappaiE Handman
Mar 8, 1996·The Journal of Biological Chemistry·P J Casey, M C Seabra
Oct 25, 1996·The Journal of Biological Chemistry·J W EricksonR A Cerione
Jun 26, 1999·Biochimica Et Biophysica Acta·A Oliff
Aug 26, 1999·Parasitology Today·M C FieldH Field
Feb 17, 2000·Gene·S M Mauricio de MendoncaU Gazos Lopes
Oct 20, 2000·Experimental Parasitology·S T LealE Rondinelli
Jan 12, 2001·Physiological Reviews·Y TakaiT Matozaki
Feb 27, 2001·Nucleic Acids Research·F RiveroA A Noegel
May 22, 2001·The Journal of Biological Chemistry·J L Nepomuceno-SilvaU G Lopes
Jun 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·L PiacenzaR Radi
Feb 28, 2002·Current Pharmaceutical Design·Wanderley De Souza
Sep 28, 2002·Blood·Gary M Bokoch, Becky A Diebold
Dec 17, 2002·Trends in Parasitology·Gareth W MorganMark Carrington
Feb 26, 2004·Gene·José L Nepomuceno-SilvaUlisses G Lopes

❮ Previous
Next ❯

Citations

May 3, 2013·Parasites & Vectors·Ethel Bayer-SantosJosé Franco da Silveira
May 10, 2008·Trends in Parasitology·Mark C Field, Amanda J O'Reilly
Oct 10, 2006·International Journal for Parasitology·C D S MacêdoS M R Teixeira
May 13, 2006·Biochemical and Biophysical Research Communications·Luiz Dione Barbosa De MeloUlisses Gazos Lopes
Dec 13, 2005·International Journal for Parasitology·Marta T GomesAngela H Lopes
Feb 14, 2012·Biochemical and Biophysical Research Communications·Guilherme Rodrigo Reis Monteiro dos SantosUlisses Gazos Lopes
May 5, 2009·Progress in Histochemistry and Cytochemistry·Wanderley de SouzaNarcisa L Cunha-e-Silva
Jul 4, 2019·Parasitology Research·Alessandra Catarina Chagas-LimaGeorgia Correa Atella

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Biochemical and Biophysical Research Communications
Luiz Dione Barbosa de MeloUlisses Gazos Lopes
Biochemical and Biophysical Research Communications
Júlia Rolão AraripeEdson Rondinelli
Gene
José Luciano Nepomuceno-SilvaUlisses Gazos Lopes
Biochemical and Biophysical Research Communications
Júlia Rolão AraripeEdson Rondinelli
Biochemical and Biophysical Research Communications
Fabiane Pereira RamosEdson Rondinelli
© 2021 Meta ULC. All rights reserved