Tdrd1 acts as a molecular scaffold for Piwi proteins and piRNA targets in zebrafish

The EMBO Journal
Hsin-Yi HuangRené F Ketting


Piwi proteins function in an RNAi-like pathway that silences transposons. Piwi-associated RNAs, also known as piRNAs, act as a guide to identify Piwi targets. The tudor domain-containing protein Tdrd1 has been linked to this pathway but its function has thus far remained unclear. We show that zebrafish Tdrd1 is required for efficient Piwi-pathway activity and proper nuage formation. Furthermore, we find that Tdrd1 binds both zebrafish Piwi proteins, Ziwi and Zili, and reveals sequence specificity in the interaction between Tdrd1 tudor domains and symmetrically dimethylated arginines (sDMAs) in Zili. Finally, we show that Tdrd1 complexes contain piRNAs and RNA molecules that are longer than piRNAs. We name these longer transcripts Tdrd1-associated transcripts (TATs). TATs likely represent cleaved Piwi pathway targets and may serve as piRNA biogenesis intermediates. Altogether, our data suggest that Tdrd1 acts as a molecular scaffold for Piwi proteins, bound through specific tudor domain-sDMA interactions, piRNAs and piRNA targets.


Jul 30, 1992·Nature·A Ephrussi, R Lehmann
Aug 3, 2002·Genes & Development·Jason R KennerdellRichard W Carthew
Jan 23, 2004·Development·Satomi Kuramochi-MiyagawaToru Nakano
Jun 16, 2004·Developmental Biology·Anne Vatland Krøvel, Lisbeth Charlotte Olsen
Apr 29, 2005·Cell Research·Travis Thomson, Paul Lasko
Jun 16, 2005·The Journal of Biological Chemistry·Jocelyn Côté, Stéphane Richard
Jun 6, 2006·Nature·Angélique GirardMichelle A Carmell
Jun 6, 2006·Nature·Alexei AravinThomas Tuschl
Jun 17, 2006·Science·Nelson C LauRobert E Kingston
Jul 1, 2006·Science·Vasily V VaginPhillip D Zamore
Oct 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shinichiro ChumaNorio Nakatsuji
Mar 27, 2007·Nature Structural & Molecular Biology·Yohei Kirino, Zissimos Mourelatos
Mar 31, 2007·Developmental Cell·Michelle A CarmellGregory J Hannon
Apr 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ai Khim Lim, Toshie Kai
Oct 4, 2008·The EMBO Journal·Saskia HouwingRené F Ketting
Jan 17, 2009·Nature Reviews. Genetics·Megha Ghildiyal, Phillip D Zamore
Feb 24, 2009·Journal of Molecular Biology·Anders FribergMichael Sattler
Apr 7, 2009·Current Biology : CB·Jianquan WangHaifan Lin
Apr 21, 2009·Nature Cell Biology·Yohei KirinoZissimos Mourelatos
Aug 29, 2009·The EMBO Journal·Nelson C LauMichael D Blower
Nov 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Chen ChenTony Pawson
Nov 21, 2009·Current Pharmaceutical Design·J M MatthewsJ P Mackay
Dec 17, 2009·PLoS Genetics·Alexei A AravinAlex Bortvin
Jan 19, 2010·The Journal of Biological Chemistry·Yohei KirinoZissimos Mourelatos

❮ Previous
Next ❯


Dec 22, 2012·Nucleic Acids Research·Shinpei KawaokaSusumu Katsuma
Jun 21, 2014·Integrative and Comparative Biology·Josien C van Wolfswinkel
Sep 27, 2014·Proceedings of the National Academy of Sciences of the United States of America·Aisa SakaguchiShawn Ahmed
Nov 22, 2014·Critical Reviews in Biochemistry and Molecular Biology·Roland Dosch
Nov 20, 2015·Seminars in Cell & Developmental Biology·Robyn S M Lim, Toshie Kai
Sep 27, 2012·Molecular Reproduction and Development·Ming Gao, Alexey L Arkov
Sep 22, 2012·Stem Cells·Shiva BamezaiChristian Buske
May 18, 2016·Developmental and Comparative Immunology·Xiao-Wu ChenYi-Feng Gu
Jun 8, 2017·International Journal of Molecular Sciences·Xiangyan DaiZhan Yin
Jul 1, 2017·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Feng ChenRongjia Zhou
Feb 27, 2016·F1000Research·Florence Marlow
Jul 30, 2018·Fish Physiology and Biochemistry·Xiaowu ChenJinliang Zhao
Nov 18, 2018·Nature Reviews. Genetics·Deniz M OzataPhillip D Zamore
Nov 28, 2020·Development Genes and Evolution·Yao-Ting DongGao-Feng Qiu
Jun 7, 2012·Development·Jun Wei PekToshie Kai
Aug 28, 2021·Animals : an Open Access Journal From MDPI·Ji-Hye YoonHan-Kyu Lim
Sep 10, 2021·Laboratory animal research·Chong Pyo ChoeHyunju Ro

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.