TdTomato and EGFP identification in histological sections: insight and alternatives

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
L M MorrisT M Crombleholme

Abstract

Tandem dimer Tomato (tdTomato) provides a useful alternative to enhanced green fluorescent protein (eGFP) for performing simultaneous detection of fluorescent protein in histological sections together with fluorescence immunohistochemistry (IHC). eGFP has many properties that make it useful for cell labeling; however, during simultaneous fluorescence IHC, the usefulness of eGFP may be limited. This limitation results from a fixation step required to identify eGFP in histological tissue sections that can mask antibody epitopes and adversely affect staining intensity. An alternative fluorescent protein, tdTomato, may assist concurrent detection of fluorescent protein within tissue sections and fluorescence IHC, because detection of tdTomato does not require tissue fixation. Tissue sections were obtained from various organs of mice ubiquitously expressing eGFP or tdTomato that were either unfixed or fixed with 4% paraformaldehyde. These tissues later were combined with fluorescence IHC. Both eGFP and tdTomato displayed robust signals in fixed frozen sections. Only tdTomato fluorescence, however, was detected in unfixed frozen sections. Simultaneous detection of fluorescence IHC and fluorescent protein in histological sections was ...Continue Reading

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Citations

Nov 28, 2012·Journal of the American Society for Mass Spectrometry·Kamila ChughtaiKristine Glunde
Nov 20, 2013·Experimental Eye Research·Kumar SukhdeoJeremy N Rich
Mar 2, 2018·Tissue Engineering. Part C, Methods·Brian C SyverudLisa M Larkin
Sep 6, 2020·Scientific Reports·Valentina ScandellaMarlen Knobloch
May 18, 2019·Communications Biology·Andrew ChojnackiKamala D Patel
Jan 12, 2020·Communications Biology·Andrew ChojnackiKamala D Patel
Jun 22, 2021·Frontiers in Cellular and Infection Microbiology·María Fernanda García-BustosPaola Andrea Barroso

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