Tea polyphenols protect bovine mammary epithelial cells from hydrogen peroxide-induced oxidative damage in vitro

Journal of Animal Science
Yanfen MaJ J Loor

Abstract

Periparturient dairy cows are subjected to altered intracellular reduction-oxidation (redox) balance due to the high metabolic rates and physiological adaptations characteristic of the transition into lactation. Such conditions could alter oxidative stress status. The objective of this study was to investigate the cytoprotective effects of tea polyphenols (TP) in cultured bovine mammary epithelial cells (BMEC) exposed to hydrogen peroxide (H2O2)-induced oxidative stress. To establish oxidative stress, isolated BMEC were exposed to increasing concentrations of H2O2 (0, 100, 200, 400, 600, 800, and 1,000 µM) for 0, 2, 4, 6, 8, 12, and 24 h. Doses of TP (0, 60, 80, and 100 µg/mL) were evaluated by pretreatment of BMEC for 0, 2, 4, 6, 8, 12 and 24 h, followed by an H2O2 (600 µM per culture well) challenge for 6 h. Bovine mammary epithelial cells were preincubated for 30 min with or without 2,4-dinitrochloro-benzene (DNCB), then cultured with or without TP (100 µg/mL) for another 12 h followed by H2O2 (600 µM per culture well) exposure. There were 5 replicate cultures for each treatment in each experiment. Treatment with 600 µM H2O2 per culture well for 6 h induced oxidative damage of BMEC, indicating this system could be used to es...Continue Reading

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Citations

Jul 31, 2020·Dose-response : a Publication of International Hormesis Society·Edward J CalabreseVittorio Calabrese
Nov 5, 2020·Animals : an Open Access Journal From MDPI·Laura MenchettiGabriele Brecchia

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